Although it is well known that the function of MyoG cannot be compensated for other MRFs, the molecular mechanism by which MyoG controls muscle cell differentiation is still unclear. Therefore, in this study, RNA-Seq technology was applied to profile changes in gene expression in response to MyoG knock-down (MyoGkd) in primary bovine muscle satellite cells (MSCs). This study is the first RNA-Seq based gene expression analysis of MyoGkd undertaken in primary bovine MSCs.
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Sex, Specimen part, Disease
View SamplesTo provide novel insights into the molecular basis of floral initiation, RNASeq was used to characterize the soybean transcriptome of leaf and micro-dissected shoot apical meristem at different time points after short-day treatment.
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View Samplesthe mRNA expression of pig lung tissue. there are four groups: LW pigs infected with PCV2, LW pigs control, Yorkshire × Landrace (YL) pigs infected with PCV2, Yorkshire × Landrace (YL) pigs control.
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Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment
View SamplesTotal RNA of thymus from mouse different development stages were isolated and deeply sequenced by SAPAS to investigated the global gene regualtion events during thymus genesis and maturation.
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View SamplesRectal cancer stage II carcinoma and adjacent normal tissue transcriptome
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View SamplesTo compare the transcriptomes of wild-type and sma mutants, three repeats of sma-4(rax3), sma-2(rax5) and out-crossed wild-type sibling C.elegans were synchronized and grown to day-1 adults then RNA-sequenced.
No associated publication
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Sex, Specimen part, Cell line
View SamplesMaize exhibits levels of structural variation (SV) of non-repeat sequences that are unprecedented among higher eukaryotes. This SV includes hundreds of copy number variants (CNVs) and thousands of presence/absence variants (PAVs). Many of the PAVs contain intact, expressed, single-copy genes that are present in one haplotype but absent from another. The goal of this project is to test the hypothesis that differences in gene copy number (both gains and losses) contribute to the extraordinary phenotypic diversity and plasticity of maize. Maize is a good model for these studies because it exhibits a rapid decay of linkage disequilibrium (LD) and because a draft genome sequence of the B73 inbred and mapping populations are available. As a first step, the "Zeanome", a near-complete set of genes present in B73, other maize lines and the wild ancestor of maize (teosinte), is being defined using transcriptomic data.
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View SamplesNo description.
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Cell line
View SamplesRNA-seq transcriptome profiles of genetically fate-mapped serotonin neurons, manually sorted from multiple anatomic domains, at both population and single cell resolution.
No associated publication
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Sex, Specimen part, Cell line
View SamplesHeterosis which is the improved vigor of F1-hybrids compared to their parents is widely exploited in maize (Zea mays L.) breeding to produce elite hybrids of superior yield. The transcriptomes of the maize inbred lines B73 and Mo17 and their reciprocal hybrid offspring were surveyed in the meristematic zone, the elongation zone, cortex and stele tissues of primary roots, prior to the developmental manifestation of heterosis. Single parent expression (SPE) is consistent with the dominance model for heterosis in that it denotes genes that are expressed in only one parent but in both reciprocal hybrids. In primary root tissues, between 1,027 (elongation zone) and 1,206 (stele) SPE patterns were observed. As a consequence, hybrids displayed in each tissue >400 active genes more than either parent. Analysis of tissue-specific SPE dynamics revealed that 1,233 of 2,233 SPE genes displayed SPE in all tissues in which they were expressed while 1,000 SPE genes displayed in at least one tissue a non-SPE pattern. In addition, 64% (17,351/ 27,164) of all expressed genes were assigned to the two subgenomes which are the result of an ancient genome duplication. By contrast, only between 18 and 25% of the SPE genes were assigned to a subgenome suggesting that a disproportionate number of SPE genes are evolutionary young and emerged after genome duplication. We hypothesize that this phenomenon is associated with human selection of favorable maize genotypes which might primarily affect younger genes rather than genes whose functions have been conserved for millions of years.
Nonsyntenic genes drive highly dynamic complementation of gene expression in maize hybrids.
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View Samples