Description

The acute myeloblastic leukemia cell line HL-60 can be differentiated in vitro from a rapidly growing promyelocytic form to a nongrowing form, resembling neutrophils with segmented (lobulated) nuclei, by the addition of retinoic acid. Treating the undifferentiated cells with phorbol ester (TPA) leads to rapid cessation of cell division and attachment of the treated cells to culture dishes, exhibiting characteristics of macrophage. The rapidly differentiating subline HL-60/S4 has been used to study nuclear shape, chromatin structure and cytoskeletal changes during differentiation induced by RA and TPA. This study seeks to understand phenotypic properties of the differentiated granulocyte and macrophage forms of HLA-60/S4 by examining through RNA-Seq the transcriptional differences between RA- TPA- and untreated cells 4 days after exposure.

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