github link
Accession IconSRP066625

Comparing The Transcriptomes of Granulocytic and Macrophage Differentiated Forms of HL-60/S4 Cells

Organism Icon Homo sapiens
Sample Icon 12 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

Submitter Supplied Information

The acute myeloblastic leukemia cell line HL-60 can be differentiated in vitro from a rapidly growing promyelocytic form to a nongrowing form, resembling neutrophils with segmented (lobulated) nuclei, by the addition of retinoic acid. Treating the undifferentiated cells with phorbol ester (TPA) leads to rapid cessation of cell division and attachment of the treated cells to culture dishes, exhibiting characteristics of macrophage. The rapidly differentiating subline HL-60/S4 has been used to study nuclear shape, chromatin structure and cytoskeletal changes during differentiation induced by RA and TPA. This study seeks to understand phenotypic properties of the differentiated granulocyte and macrophage forms of HLA-60/S4 by examining through RNA-Seq the transcriptional differences between RA- TPA- and untreated cells 4 days after exposure.
PubMed ID
No associated PubMed ID
Publication Title
No associated publication
Total Samples
Submitter’s Institution
No associated authors
Alternate Accession IDs


Show of 0 Total Samples
Accession Code
Processing Information
Additional Metadata
No rows found