Groups of adult zebrafish (9 male and 9 female) were exposed for 7 days to 50 ng/L (168.7 pmol/L) of 17a-ethinylestradiol (EE2). Transcriptome response of EE2 in zebrafish liver were analysed.
No associated publication
None
No sample metadata fields
View SamplesWe report the biological activity of a Py-Im polyamide targeted to the sequence 5'-WGWWCW-3', which is found in a subset of ARE half-sites. This molecule reduces the growth of enzalutamide-resistant LREX' cells, both in vitro and in vivo. Gene expression changes associated with polyamide treatment in both settings are deposited here.
No associated publication
None
Sex, Specimen part, Cell line
View SamplesVCaP cells were treated with 10uM of a Py-Im polyamided targeted to the DNA sequence 5''-WGWWCW-3'' for 24hrs. Gene expression changes are normalized to untreated VCaP cells.
No associated publication
None
Sex, Age, Specimen part, Cell line
View SamplesRNA was isolated from colorectal cancer (HCT116) and normal colon cells (HCoEpic) treated with toxic agents (bisphenol A (BPA), hexabromocyclododecane (HBCD), 4-tert-octylphenol (OP)) or Vehicle (DMSO) and then preformed NGS using Illumina protocol.
No associated publication
None
Sex, Specimen part, Disease, Cell line, Treatment
View SamplesThe study aimed to investigate genome-wide transcriptome changes in response to L-lactate in primary neuron cultures.
No associated publication
None
Sex, Specimen part, Cell line, Treatment
View SamplesEffect of ethanol or nicotine exposure on gene expression compared to control. Duplicate arrays from ethanol or nicotine treated animals compared with triplicate arrays from paired control animals. In total 4 treatment arrays (2 ethanol, 2 nicotine) and 3 control arrays (from control animals treated in parallel with ethanol-treated fish and nicotine-treated fish.)
Gene expression changes in a zebrafish model of drug dependency suggest conservation of neuro-adaptation pathways.
None
Specimen part, Compound
View SamplesTranscriptomic sequencing of human gastric cancer cell line (AGS) upon citral treatment
No associated publication
None
Sex, Specimen part, Disease, Cell line
View SamplesThis project examined the effects of substrate stress relaxation, stiffness, and adhesion ligand density on the D1 mouse MSC cell line. Cells were cultured in alginate hydrogels with low and high values for each of these material parameters for 40 hours before isolation and sequencing.
No associated publication
None
Sex, Specimen part, Cell line, Treatment
View SamplesGene expression profiling analysis reveals ?-3 polyunsaturated fatty acids attenuates a high fat diet induced fatty liver
No associated publication
None
No sample metadata fields
View SamplesDengue virus (DENV) infection causes profound changes in the host cells and these changes underlie the immune response-based viral clearance and pathogenesis. There are several major cell/tissue types relevant for DENV pathogenesis in vivo, including immune cells, liver, and vascular endothelial cells. We applied a directed differentiation system that produces hepatocyte-like cells (HLCs) from pluripotent stem cells to investigate various aspects of DENV- hepatic cells interaction. Human embryonic stem cells were resistant to DENV infection while progeny hepatic cells were permissive. The transition to DENV permissiveness coincided with the upregulation of entry factors for the virus. Infection of HLCs by DENV was self-limiting due to the activation of the interferon (IFN) pathways, which protected by-stander cells from infection but failed to induce the same level of interferon-induced genes (ISGs) expression in the infected cells due to the subversion of IFN signaling by DENV. Innate immunity also protected the infected cells from virus-induced apoptosis. Furthermore, DENV infection activated the NF-?B pathway, increased production of reactive oxidative species (ROS), and led to production of inflammatory cytokines which may contribute to the cytokine storm implicated in dengue hemorrhagic fever (DHF). Finally, DENV infection of HLCs resulted several in vitro phenotypes that may have relevance for acute liver failure and vascular permeability during DHF. These include the disruption of adherens junctions and the downregulation of many liver specific genes such as albumin (ALB) and coagulation factor V (F5).
No associated publication
None
Sex, Age, Specimen part, Disease, Cell line
View Samples