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accession-icon GSE45220
Expression data from HT-29, a human colonic epithelial cell line
  • organism-icon Homo sapiens
  • sample-icon 9 Downloadable Samples
  • Technology Badge Icon Affymetrix Human Gene 1.1 ST Array (hugene11st)

Description

Gene(s) in epithelial cells can be upregulated or downregulated by different stimuli in a tissue-specific manner. We used genome-wide microarray analysis to profile the expression of genes in HT-29 cells that are upregulated after stimulation with sodium butyrate (NaB) and subsequently downregulated by the addition of ciprofloxacin antibiotic. The specific aim was to evaluate genes that are associated with mucosal immunity.

Publication Title

No associated publication

Alternate Accession IDs

E-GEOD-45220

Sample Metadata Fields

Specimen part, Cell line, Treatment

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accession-icon SRP148483
Transcriptome data of flower bud distortion in witches' broom disease of soybean (Glycine max) variety JS-335 (R5 stage)
  • organism-icon Glycine max
  • sample-icon 2 Downloadable Samples
  • Technology Badge IconIllumina HiSeq 2000

Description

No description.

Publication Title

No associated publication

Alternate Accession IDs

None

Sample Metadata Fields

Specimen part

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accession-icon E-MTAB-6829
Microarray and transcript expression analysis of P. aeruginosa grown with or without bacterial (Exiguobacterium indicum SJ16) extracts
  • organism-icon Pseudomonas aeruginosa pao1
  • sample-icon 2 Downloadable Samples
  • Technology Badge Icon Affymetrix Pseudomonas aeruginosa Array (paeg1a)

Description

Differential expression of regulatory genes of reference strain P. aeruginosa PA01, involved in the quorum sensing was analyzed using Microarray. Total RNA was isolated from reference strain P. aeruginosa PA01, grown with or without bacterial extracts (1.0 mg/ml) using TRI reagent. Total RNA was quantified, and 10 µg RNA was converted to cDNA, fragmented and labelled by following GeneChip P. aeruginosa PA01 genome array user manual. Labelled cDNAs were hybridized with P. aeruginosa genome array gene chip, washed and stained. Hybridized chips were scanned, processed and analyzed using expression console and transcriptome analysis console.

Publication Title

No associated publication

Alternate Accession IDs

None

Sample Metadata Fields

No sample metadata fields

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refine.bio is a repository of uniformly processed and normalized, ready-to-use transcriptome data from publicly available sources. refine.bio is a project of the Childhood Cancer Data Lab (CCDL)

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Cite refine.bio

Casey S. Greene, Dongbo Hu, Richard W. W. Jones, Stephanie Liu, David S. Mejia, Rob Patro, Stephen R. Piccolo, Ariel Rodriguez Romero, Hirak Sarkar, Candace L. Savonen, Jaclyn N. Taroni, William E. Vauclain, Deepashree Venkatesh Prasad, Kurt G. Wheeler. refine.bio: a resource of uniformly processed publicly available gene expression datasets.
URL: https://www.refine.bio

Note that the contributor list is in alphabetical order as we prepare a manuscript for submission.

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