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SRP076532
Homo sapiens
46 Downloadable Samples
Ion Torrent Proton
Description
Human monocytes were infected with three different pathogens. Furthermore, some of the samples were additionally treated with either vitamin A or D. The goal of the study is to identify different gene regulatory patterns, e.g. to figure out the impact of the vitamin treatment upon pathogenic stimulus.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Treatment
View Samples- Homo sapiens
- 44 Downloadable Samples
- Illumina HiSeq 2000
Description
A549 cells that had been transfected 48 hours earlier with siRNA to MDA5 or non-specific negative control siRNA, were uninfected or infected for 6, 12, 24, and 48 hours with HRV-B14 or RSV. For each time point, infections were performed in triplicate. Total cellular RNA were isolated using RNeasy Mini Kit (Qiagen). Multiplexed RNA libraries were prepared using the Truseq RNA sample prep kit (Illumina). Libraries were sequenced on a HiSeq 2000 Sequencing System (Illumina) to produce 50 bp single end reads. Sequencing reads were aligned with ELAND to the human reference genome version hg19.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line, Treatment
View Samples