Col-0 and WEE1KO (wee1-1 and wee1-2) were germinated on control medium on a nylon mesh and transferred 5 days after germination to control medium. Samples were harvested at three different time points after transfer: 0 h, 5 h, and 24 h. All sampling points were performed in four independent experiments for Col-0, two independent experiments for wee1-1 and two independent experiments for wee1-2. For each time point in each experiment ᄆ50 root tips were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with TriZol reagent (Invitrogen) and purified with RNneasy kit (Qiagen). The RNA of two independent experiments for Col-0 and WEE1KO were subsequently pooled and used for microarray analysis.
The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress
None
Age, Specimen part, Time
View SamplesCol-0 and WEE1KO (wee1-1 and wee1-2) were germinated on control medium on a nylon mesh and transferred 5 days after germination to medium supplemented with 2 mM HU. Samples were harvested at three different time points after transfer: 0 h, 5 h, and 24 h. All sampling points were performed in four independent experiments for Col-0, two independent experiments for wee1-1 and 2 independent experiments for wee1-2. For each time point in each experiment ᄆ50 root tips were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with TriZol reagent (Invitrogen) and purified with RNneasy kit (Qiagen). The RNA of two independent experiments for Col-0 and WEE1KO were subsequently pooled and used for<br></br>microarray analysis.
The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress
None
Age, Specimen part, Time
View SamplesTranscriptome changes 1h or 4h following DELLA stabilisation in microdissected fully proliferating Arabidopsis leaves
Gibberellins and DELLAs: central nodes in growth regulatory networks.
Specimen part, Treatment, Time
View SamplesTranscriptome changes in 35S::KLU shoots at stage 1.03 compared to wild-type (WT) Col-0 shoots
No associated publication
Specimen part
View SamplesThe transcriptional response of Arabidopsis thaliana cell suspensions following treatment with the stress hormone methyl jasmonate (MeJA) was monitored over time 16 hours after subcultivation. Three time points were included: 30 minutes, 2 hours and 6 hours after elicitation with 50µm MeJA or DMSO as a control.
Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells.
None
Compound, Time
View SamplesMassively parallel sequencing of human urinary exosome/microvesicle RNA reveals a predominance of non-coding RNA
No associated publication
None
No sample metadata fields
View SamplesRNA-SEQ of Arabidopsis thaliana leaf tissues under normal and low CO2
No associated publication
None
Specimen part
View SamplesNo description.
No associated publication
None
Sex, Specimen part, Cell line
View SamplesHuman rhinovirus and influenza virus infections of the upper airway lead to colds and the flu and can trigger exacerbations of lower airway diseases including asthma and chronic obstructive pulmonary disease. Despite modest advances in the diagnosis and treatment of infections by these viruses, novel diagnostic and therapeutic targets are still needed to differentiate between the cold and the flu, since the clinical course of influenza can be severe while that of rhinovirus is usually more mild.
A systems approach to understanding human rhinovirus and influenza virus infection.
Time
View SamplesAssessment of mRNA expression levels in fat biopsies from subcutaneous adipose tissue from unrelated individuals.
A systems genetics approach implicates USF1, FADS3, and other causal candidate genes for familial combined hyperlipidemia.
Specimen part
View Samples