Three-day-old wild-type Col-0 plants grown on filter paper in the dark, as described above, were exposed to blue light and harvested after 1 hour exposure. Total RNAs were extracted using Trizol reagent (Life Technologies) and purified by PureLink RNA Mini Kits (Life Technologies). Directional RNA-seq libraries were constructed using TruSeq Small RNA Sample Prep Kits and TruSeq RNA Sample Preparation Kits according to the Directional mRNA-Seq Library Prep. Manual (Illumina) and sequenced using a HiSeq sequencer (Illumina).
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Age, Specimen part, Treatment
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View SamplesRNA-seq of Babesia microti isolates and rodent host
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View SamplesRNA-seq of Babesia microti isolates and rodent host
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View SamplesRNA-seq of Babesia microti isolates and rodent host
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View SamplesRNA-seq of Babesia microti isolates and rodent host
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No sample metadata fields
View SamplesRNA-seq of Babesia microti isolates and rodent host
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View SamplesRNA-seq of Babesia microti isolates and rodent host
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No sample metadata fields
View SamplesThe goal of these libraries is to find transcriptome signatures of the different phases of the cell cycle. Single HeLa cells expressing fluorescent reporters that peak in G1 and G2/M phases were captured in C1 Single-Cell Auto Prep systems (Fluidigm recording red and green fluorescence for each cell individually, each cell was lysed and their RNAs converted to cDNAs in the C1 systems using SMARTer kits (Clontech). The cDNAs were then collected and converted to RNA-seq libraries by tagmentation using Illumina/Nextera kits. The cells used in this experiment are unpublished as of today, and precise details will be given later, together with the fluorescence values. In the meantime, this dataset can be used to assess reproducibility of single-cell RNA-seq over 5 runs in the Fluidigm system.
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View SamplesBmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation after 24 hrs cells were infected with mycobacterium tuberculosis to identify the host factors involved in infection.
IL-4Rα-dependent alternative activation of macrophages is not decisive for Mycobacterium tuberculosis pathology and bacterial burden in mice.
Sex, Specimen part
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