A model system of Potyvirus turnip mosaic virus and Arabidopsis was used in this experiment. GFP-tagged virus supplied a visualized marker for us to localize the viral infection foci and its expansion on leaf under UV light. Initially, we dissect an individual infection focus and its adjacent region into four parts and define those four parts as zone 0, 1, 2, and 3, which represented different viral infection stages respectively. Corresponding fours parts were also dissected from control plant treated with turnip leaf sap only. This process was replicated three times totally.
Spatial analysis of arabidopsis thaliana gene expression in response to Turnip mosaic virus infection.
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Age, Specimen part
View SamplesA large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13).
Interaction-dependent gene expression in Mla-specified response to barley powdery mildew.
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Age, Specimen part, Disease, Disease stage, Cell line, Time
View SamplesTime-course analysis of shade responsive genes in Col and 12 mutants.
No associated publication
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Specimen part, Treatment
View SamplesTime-course data of shade avoidance in NAM parents
No associated publication
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Specimen part, Treatment
View SamplesDevelopmental gradient of expanding maize leaf
No associated publication
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Age, Specimen part
View SamplesIn order to study the gene expression of pollen tubes as they grow in silk after pollination, we pollinated maize W22 silks with maize B73 pollen. The recent (2016) advent of the W22 genome assembly and annotation allows us to single out RNA-seq reads originating from the pollen tubes. B73 pollen, W22 silk and B73 seedling controls were sequenced as well.
No associated publication
None
Specimen part
View SamplesGlycine max and Phytophthora sojae infected Glycine max Transcriptome
No associated publication
None
Specimen part
View SamplesThe project was aim of search the different mechanism of resoonse to soybean cyst nematode and mining the candidate resisitance genes from next generation sequencing
No associated publication
None
Specimen part, Disease, Disease stage
View SamplesPowdery mildew, caused by the fungus Blumeria graminis (DC) Speer, is one of the most important foliar diseases of cereals worldwide. It is an obligate biotrophic parasite, colonising leaf epidermal cells to obtain nutrients from the plant cells without killing them. Syringolin A (sylA), a circular peptide secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers a hypersensitive cell death reaction (HR) at infection sites when sprayed onto powdery mildew infected wheat which essentially eradicates the fungus. The rational was to identify genes whose expression was specifically regulated during HR, i.e. genes that might be involved in the switch of compatibility to incompatibility.<br></br>Powdery mildew-infected or uninfected plants were treated with syringolin two days after infection and plant material for RNA extraction was collected at 0.5, 1, 2, 4, 8, 12 hours after treatment (hat), resulting in an early (2 and 4 hat) and late pool (8 and 12 hat). Plant material that was uninfected prior to syringolin treatment was collected 8 and 12 hat (late pool of uninfected plant material), and 1 hat, respectively.
Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites.
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Compound, Time
View SamplesThe transcriptional response of Arabidopsis thaliana cell suspensions following treatment with the stress hormone methyl jasmonate (MeJA) was monitored over time 16 hours after subcultivation. Three time points were included: 30 minutes, 2 hours and 6 hours after elicitation with 50µm MeJA or DMSO as a control.
Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells.
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Compound, Time
View Samples