Gene expression profiling leading to the identification of novel components in the EDS1/PAD4-regulated defence pathway
Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7.
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Age, Specimen part, Time
View SamplesIn this experiment, we compared transcriptome of 2 homozygous knock out mutants in the CLV1 gene (clv1-12 and clv1-13) to their wild type Ws-2 when challenged with the virulent Rd15 strain of Ralstonia solanacearum. The two mutants show a significant delay of symptom appearance. The experiment was conducted on roots and leaves of 4 week old plants harvested at the time of inoculation and when the first symptoms appeared on the susceptible wild type (4 days after inoculation).
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Specimen part, Disease stage
View SamplesTime-course analysis of shade responsive genes in Col and 12 mutants.
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Specimen part, Treatment
View SamplesTime-course data of shade avoidance in NAM parents
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Specimen part, Treatment
View SamplesDevelopmental gradient of expanding maize leaf
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Age, Specimen part
View SamplesA model system of Potyvirus turnip mosaic virus and Arabidopsis was used in this experiment. GFP-tagged virus supplied a visualized marker for us to localize the viral infection foci and its expansion on leaf under UV light. Initially, we dissect an individual infection focus and its adjacent region into four parts and define those four parts as zone 0, 1, 2, and 3, which represented different viral infection stages respectively. Corresponding fours parts were also dissected from control plant treated with turnip leaf sap only. This process was replicated three times totally.
Spatial analysis of arabidopsis thaliana gene expression in response to Turnip mosaic virus infection.
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Age, Specimen part
View SamplesPro35SLBD16:GR or Pro35SLBD18:GR transgenic seedlings that overexpress LBD16 or LBD18 fused to glucocorticoidsteroid hormone binding domain(GR) under CaMV35S promoter were grown for 12 days under long-day conditions (16h light/ 8h dark).
LBD18 acts as a transcriptional activator that directly binds to the EXPANSIN14 promoter in promoting lateral root emergence of Arabidopsis.
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Specimen part, Compound, Time
View SamplesSoybean transcript fluctuations were observed in response to Rhizoctonia solani AG-1 IA causing Rhizoctonia foliar blight. The overall goal was to observe the general transcriptome fluctuations using RNAseq Illumina HiSeq analysis.
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Specimen part, Disease
View SamplesIn order to study the gene expression of pollen tubes as they grow in silk after pollination, we pollinated maize W22 silks with maize B73 pollen. The recent (2016) advent of the W22 genome assembly and annotation allows us to single out RNA-seq reads originating from the pollen tubes. B73 pollen, W22 silk and B73 seedling controls were sequenced as well.
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Specimen part
View Samplesdifferential expression between wild-type pistils of Arabidopsis thaliana at late 11 to late 12 floral stages, and similar stage pistils of coatlique mutant which lacks a functional embryo sac
Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte.
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Specimen part
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