LCT-EC001 inoculated under stimulating microgravity in semi-solid BHI medium (Brain Heart Infusion + 0.6% low-melting agarose (invitrogen)) for about 15 days, at the same temperature intra the spacecraft.
No associated publication
None
Specimen part, Cell line
View SamplesEarly embryo RNA-seq sequencing
No associated publication
None
Sex, Specimen part
View SamplesTranscriptome analysis of total RNA samples from HEK293-PIGS-KO and HEK293-PIGS-UBE2J1-DKO cells. To check whether KO of UBE2J1 upregulates genes of GPI biosthesis pathway, we used microarrays to analyze gene expression change by KO of UBE2J1 and comfirmed that known GPI pathway genes are not changed by ERAD-deficiency.
Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.
Cell line
View SamplesTest effects of mtDNA variation on nuclear transcript expression using various mtDNA haplotypes on isogenic nuclear backgrounds
No associated publication
None
Sex, Age, Specimen part, Cell line
View SamplesMitonuclear transcriptomics
No associated publication
None
Sex, Age, Specimen part, Cell line, Treatment
View SamplesPseudomonas fluorescens strain SS101 (Pf.SS101) promotes growth of Arabidopsis thaliana, enhances greening and lateral root formation, and induces systemic resistance (ISR) against the bacterial pathogen Pseudomonas syringae pv. tomato (Pst). Here, targeted and untargeted approaches were adopted to identify bacterial determinants and underlying mechanisms involved in plant growth promotion and ISR by Pf.SS101. Based on targeted analyses, no evidence was found for volatiles, lipopeptides and siderophores in plant growth promotion by Pf.SS101. Untargeted, genome-wide analyses of 7,488 random transposon mutants of Pf.SS101 led to the identification of 21 mutants defective in both plant growth promotion and ISR. Many of these mutants, however, were auxotrophic and impaired in root colonization. Genetic analysis of three mutants followed by site-directed mutagenesis, genetic complementation and plant bioassays revealed the involvement of the phosphogluconate dehydratase gene edd, the response regulator gene colR and the adenylsulfate reductase gene cysH in both plant growth promotion and ISR. Subsequent comparative plant transcriptomics analyses strongly suggest that modulation of sulfur assimilation, auxin biosynthesis and transport, steroid biosynthesis and carbohydrate metabolism in Arabidopsis are key mechanisms linked to growth promotion and ISR by Pf.SS101.
Genome-wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance by Pseudomonas fluorescens.
Specimen part, Treatment
View SamplesTo understand the function of soybean endosperm
No associated publication
None
Specimen part
View SamplesWe identified an endothelial progenitor/stem like population in the endothelial fraction of preexsiting blood vessels.
No associated publication
Age, Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Heme ameliorates dextran sodium sulfate-induced colitis through providing intestinal macrophages with noninflammatory profiles.
Specimen part, Treatment
View SamplesWe found that CFIm68, a mRNA cleavage and polyadenylation factor implicated for alternative polyadenylation site choice, was co-purified with Thoc5, a component of human THO/TREX. Microarray analysis using human HeLa cells reveals knockdown of Thoc5 affects the expression of a subset of non-heat shock genes. Notably, depletion of Thoc5 attenuated the expression of the mRNAs polyadenylated at distal, but not proximal, polyadenylation sites, which phenocopied the depletion of CFIm68.
Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I.
Cell line, Treatment
View Samples