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SRP107867
Homo sapiens
44 Downloadable Samples
Illumina HiSeq 2000
Description
A549 cells that had been transfected 48 hours earlier with siRNA to MDA5 or non-specific negative control siRNA, were uninfected or infected for 6, 12, 24, and 48 hours with HRV-B14 or RSV. For each time point, infections were performed in triplicate. Total cellular RNA were isolated using RNeasy Mini Kit (Qiagen). Multiplexed RNA libraries were prepared using the Truseq RNA sample prep kit (Illumina). Libraries were sequenced on a HiSeq 2000 Sequencing System (Illumina) to produce 50 bp single end reads. Sequencing reads were aligned with ELAND to the human reference genome version hg19.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line, Treatment
View Samples- Danio rerio
- 6 Downloadable Samples
- IlluminaHiSeq2000
Description
The zebrafish (Danio rerio) is a prominent vertebrate development model, has been extensively utilized as the pathogen-host interaction to be studied in recent years. However, the mechanisms involved in the immune response of the zebrafish to vaccine are not fully understood. For clarify the high immune relative protection in zebrafish following the immunization of the putative Edwardsiella tarda (E. tarda) live attenuate vaccine, we performed a comparative gene expression analysis of mocked and immunized zebrafish using the RNA-seq technology and DEGseq to identify differential expressed genes, chiefly for gaining deep insight into the liver immunogenetics after WEDplas vaccinated zebrafish.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 46 Downloadable Samples
- Ion Torrent Proton
Description
Human monocytes were infected with three different pathogens. Furthermore, some of the samples were additionally treated with either vitamin A or D. The goal of the study is to identify different gene regulatory patterns, e.g. to figure out the impact of the vitamin treatment upon pathogenic stimulus.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Treatment
View Samples- Homo sapiens
- 4 Downloadable Samples
- IonTorrentProton
Description
The study aims to analysis transcriptome profiles of NiO nanoparticles (NiO NPs) on HepG2 cells. The 100, 25 and 5 ug/ml concentrations of NiO NPs were used to dose the HepG2 cells, respectively
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 24 Downloadable Samples
Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Although extensive studies have demonstrated the gene expression patterns of antigen-specific CD4+ and CD8+ T-cells during chronic hepatitis C virus (HCV) infection, the transcriptional profiles of global CD4+ and CD8+ T-cells remains unclear. In this report, we recruited 10 long-term (~20 years) treatment-nave chronic HCV (CHC) patients and 5 healthy donors (HDs) to investigate differences in global CD4+ and CD8+ T-cells gene expression profile.
Publication Title
T lymphocytes from chronic HCV-infected patients are primed for activation-induced apoptosis and express unique pro-apoptotic gene signature.
Alternate Accession IDs
Sample Metadata Fields
Specimen part
View Samples- Danio rerio
- 4 Downloadable Samples
- IlluminaHiSeq2000
Description
Groups of adult zebrafish (9 male and 9 female) were exposed for 7 days to 50 ng/L (168.7 pmol/L) of 17a-ethinylestradiol (EE2). Transcriptome response of EE2 in zebrafish liver were analysed.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina Genome Analyzer IIx
Description
The type II transmembrane serine protease, TMPRSS2, which is expressed in the epithelia of the respiratory tract and can activate varieties of respiratory viruses. We have generated TMPRSS2 knockout (KO) mice. These mice showed normal development, growth, and fertility phenotypes, compared with wild-type mice.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Danio rerio
- 188 Downloadable Samples
- Affymetrix Genechip Zebrafish ST Genome Array 1.1 (zebgene11st)
Description
Zebrafish embryos have been proposed as an attractive alternative model system for hepatotoxicity testing.
Publication Title
A transcriptomics-based hepatotoxicity comparison between the zebrafish embryo and established human and rodent in vitro and in vivo models using cyclosporine A, amiodarone and acetaminophen.
Alternate Accession IDs
Sample Metadata Fields
Compound
View Samples- Homo sapiens
- 90 Downloadable Samples
- Affymetrix HT HG-U133+ PM Array Plate (hthgu133pluspm)
Description
Here we studied the effects of anticonvulsant drug exposure in a human embryonic stem cell (hESC) based neuro- developmental toxicity test (hESTn). During neural differentiation the cells were exposed, for either 1 or 7 days, to non-cytotoxic concentration ranges of valproic acid (VPA) or carbamazepine (CBZ), anti-epileptic drugs known to cause neurodevelopmental toxicity.
Publication Title
Gene Expression Regulation and Pathway Analysis After Valproic Acid and Carbamazepine Exposure in a Human Embryonic Stem Cell-Based Neurodevelopmental Toxicity Assay.
Alternate Accession IDs
Sample Metadata Fields
Time
View Samples- Homo sapiens
- 16 Downloadable Samples
- Illumina HiSeq 4000
Description
RNA was isolated from colorectal cancer (HCT116) and normal colon cells (HCoEpic) treated with toxic agents (bisphenol A (BPA), hexabromocyclododecane (HBCD), 4-tert-octylphenol (OP)) or Vehicle (DMSO) and then preformed NGS using Illumina protocol.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line, Treatment
View Samples