A549 cells that had been transfected 48 hours earlier with siRNA to MDA5 or non-specific negative control siRNA, were uninfected or infected for 6, 12, 24, and 48 hours with HRV-B14 or RSV. For each time point, infections were performed in triplicate. Total cellular RNA were isolated using RNeasy Mini Kit (Qiagen). Multiplexed RNA libraries were prepared using the Truseq RNA sample prep kit (Illumina). Libraries were sequenced on a HiSeq 2000 Sequencing System (Illumina) to produce 50 bp single end reads. Sequencing reads were aligned with ELAND to the human reference genome version hg19.
No associated publication
None
Sex, Specimen part, Disease, Cell line, Treatment
View SamplesHuman monocytes were infected with three different pathogens. Furthermore, some of the samples were additionally treated with either vitamin A or D. The goal of the study is to identify different gene regulatory patterns, e.g. to figure out the impact of the vitamin treatment upon pathogenic stimulus.
No associated publication
None
Sex, Specimen part, Treatment
View SamplesGenerating human serotonergic neurons from fibroblasts
Generation of functional human serotonergic neurons from fibroblasts.
None
No sample metadata fields
View SamplesAnalysis of expression quantitative trait loci (eQTLs) using RNA derived from freshly harvested peripheral blood CD4+ lymphocytes from 200 asthmatics collected in clinical settings.
Mapping of numerous disease-associated expression polymorphisms in primary peripheral blood CD4+ lymphocytes.
Sex, Specimen part, Disease, Disease stage, Subject
View SamplesEx vivo and in-cell SHAPE-MaP structure probing of the mouse Xist lncRNA.
No associated publication
None
Sex, Specimen part, Cell line
View SamplesGene expression profiles in blasts from three APL patients expressing PML/RAR were assessed before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours. We then studied a U937 clone conditionally expressing PML/RAR (U937-PR), (Grignani et al. 1993) (Alcalay et al. 2003), and compared the gene expression profile prior to and after 4 hours of treatment with 1 uM RA, to that obtained from a cell line bearing an empty vector (U937-MT). For each sample, biotinylated cRNA targets were synthesized starting from 5ug of total RNA, and hybridized to the complete set of HG-U133 Affymetrix oligonucleotide chips, which explores the expression of approximately 45,000 human transcripts. Results were analyzed using MASv5 and further elaborated with the GenePicker software. GeneChip probe sets regulated by RA in each sample were clustered into non-redundant regulated genes according to UniGene release Hs.166.
Molecular signature of retinoic acid treatment in acute promyelocytic leukemia.
None
Specimen part, Disease, Cell line, Subject, Compound
View SamplesThis study provides the dectin-1 and NFAT responsive genes for 2h and 4h of curdlan treatment.
NFATc2 mediates epigenetic modification of dendritic cell cytokine and chemokine responses to dectin-1 stimulation.
Specimen part
View SamplesThe human hair follicle is not only regulated, but is a source of transcription factors, hormones, neurohormones etc. which are crucial in regulating its growth.
No associated publication
Specimen part
View SamplesComprehensive identification of rhythmically expressed genes in the mouse liver using RNA-Seq
No associated publication
None
No sample metadata fields
View SamplesNo description.
No associated publication
None
No sample metadata fields
View Samples