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accession-icon E-MTAB-3400
Response of two clavata 1 mutants to Ralstonia solanacearum
  • organism-icon Arabidopsis thaliana
  • sample-icon 23 Downloadable Samples
  • Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

Description

In this experiment, we compared transcriptome of 2 homozygous knock out mutants in the CLV1 gene (clv1-12 and clv1-13) to their wild type Ws-2 when challenged with the virulent Rd15 strain of Ralstonia solanacearum. The two mutants show a significant delay of symptom appearance. The experiment was conducted on roots and leaves of 4 week old plants harvested at the time of inoculation and when the first symptoms appeared on the susceptible wild type (4 days after inoculation).

Publication Title

No associated publication

Alternate Accession IDs

None

Sample Metadata Fields

Specimen part, Disease stage

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accession-icon E-MTAB-3402
Response of clavata 2-7 mutant to Ralstonia solanacearum
  • organism-icon Arabidopsis thaliana
  • sample-icon 16 Downloadable Samples
  • Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

Description

In this experiment, we compared transcriptome of one knock out mutants in the CLV2 gene (clv2-7) to his wild type Col-0 when challenged with the virulent GMI1000 strain of Ralstonia solanacearum. This mutant shows a significant delay of symptom appearance. The experiment was conducted on roots and leaves of 4 week old plants harvested at the time of inoculation and when the first symptoms appeared on the susceptible wild type (4 days after inoculation - 25% of wilted leaves: disease index 1, D1).

Publication Title

No associated publication

Alternate Accession IDs

None

Sample Metadata Fields

Specimen part, Disease stage

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accession-icon E-MTAB-542
Transcription profiling by array of Arabidopsis plants inccoculated with Ralstonia solanacearum hrpB strain (avirulent strain) and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain
  • organism-icon Arabidopsis thaliana
  • sample-icon 8 Downloadable Samples
  • Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

Description

Microarray analysis was performed on the aerial parts of 5 plants collected 24 hours after water treatment or inoculation by the Ralstonia solanacearum hrpB strain (avirulent strain) and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain. We compared trancriptome expression level of protected plants (hrpB prinoculation) versus non protected plants (water treatment), 24H after treatment and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain

Publication Title

Molecular Mechanisms of Biological Control on Arabidopsis thaliana / Ralstonia solanacearum pathosystem

Alternate Accession IDs

None

Sample Metadata Fields

Age, Specimen part, Subject

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accession-icon E-MEXP-152
Transcription profiling of response of adult Drosophila to oxidative and ER stress
  • organism-icon Drosophila melanogaster
  • sample-icon 7 Downloadable Samples
  • Technology Badge Icon Affymetrix Drosophila Genome Array (drosgenome1)

Description

We used oligonucleotide microarrays to address the specificities of transcriptional responses of adult Drosophila to different stresses induced by paraquat and H2O2, two oxidative stressors, and by tunicamycin which induces an endoplasmic reticulum (ER) stress. Flies were tested 24 hours after exposure to continuous stresses induced by ingestion of paraquat, H2O2 or tunicamycin at concentrations leading to similar effects on viability. We used concentrations of 1% H2O2, 5mM paraquat and 12uM of tunicamycin which lead to negligeable mortality at 24 hours. A paraquat concentration of 15mM was also used for comparison with previous studies Both specific and common responses to the three stressors were observed and whole genome functional analysis identified several important classes of stress responsive genes. Within some functional classes, we observed large variabilities of transcriptional changes between isozymes, which may reflect unsuspected functional specificities.

Publication Title

Genome wide analysis of common and specific stress responses in adult drosophila melanogaster.

Alternate Accession IDs

None

Sample Metadata Fields

Sex, Age, Compound, Time

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accession-icon E-MEXP-503
Transcription profiling by array of Arabidopsis ron2-1 knockout mutant
  • organism-icon Arabidopsis thaliana
  • sample-icon 6 Downloadable Samples
  • Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

Description

Goal:identify the genes regulated by RON2 involved in the delay of floral transitional and those associated with leaf development. The gene expression profile of mature leaf 6 of ron2-1 EMS mutant and the wild-type Ler are compared. The plant material for this experiment was grown in LEPSE-INRA Montpellier, and the microarrays at the MAF-VIB Leuven with PSB-VIB Ghent.

Publication Title

No associated publication

Alternate Accession IDs

None

Sample Metadata Fields

Specimen part

View Samples
accession-icon E-MEXP-502
Transcription profiling by array of Arabidopsis mutant for atx1
  • organism-icon Arabidopsis thaliana
  • sample-icon 6 Downloadable Samples
  • Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

Description

Analysis of the gene expression profile of the atx1 mutant of Arabidopsis thaliana compared to the wild-type, using apices tissue of in in vitro plants and Affymetrix ATH1 chips.

Publication Title

ARABIDOPSIS TRITHORAX1 dynamically regulates FLOWERING LOCUS C activation via histone 3 lysine 4 trimethylation.

Alternate Accession IDs

None

Sample Metadata Fields

Age, Specimen part

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accession-icon E-MEXP-546
Transcription profiling of Arabidopsis leading to the identification of novel components in the EDS1/PAD4-regulated defence pathwayabidopsis-Pst-eds1-pad4
  • organism-icon Arabidopsis thaliana
  • sample-icon 21 Downloadable Samples
  • Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

Description

Gene expression profiling leading to the identification of novel components in the EDS1/PAD4-regulated defence pathway

Publication Title

Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7.

Alternate Accession IDs

None

Sample Metadata Fields

Age, Specimen part, Time

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accession-icon E-MEXP-231
Transcription profiling by array of human primary lung adenocarcinomas
  • organism-icon Homo sapiens
  • sample-icon 58 Downloadable Samples
  • Technology Badge Icon Affymetrix Human Genome U133A Array (hgu133a)

Description

Gene transcription in a set of 49 human primary lung adenocarcinomas and 9 normal lung tissue samples was examined using Affymetrix GeneChip technology. We aimed to investigate differential gene expression between the two tissue types. A total of 3,442 genes, called the set MAD, were found to be either up- or down-regulated by at least two fold between the two phenotypes. Genes assigned to a particular gene ontology term were found, in many cases, to be significantly unevenly distributed between the genes in and outside MAD. Terms that were overrepresented in MAD included functions directly implicated in cancer cell metabolism. Based on their functional roles and expression profiles, genes in MAD were grouped into likely co-regulated gene sets.

Publication Title

Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays.

Alternate Accession IDs

None

Sample Metadata Fields

Sex, Age, Specimen part, Disease, Disease stage

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Cite refine.bio

Casey S. Greene, Dongbo Hu, Richard W. W. Jones, Stephanie Liu, David S. Mejia, Rob Patro, Stephen R. Piccolo, Ariel Rodriguez Romero, Hirak Sarkar, Candace L. Savonen, Jaclyn N. Taroni, William E. Vauclain, Deepashree Venkatesh Prasad, Kurt G. Wheeler. refine.bio: a resource of uniformly processed publicly available gene expression datasets.
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Note that the contributor list is in alphabetical order as we prepare a manuscript for submission.

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