This study presented the preliminary mechanistic studies of teniposide analogs for toxicity reduction
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Sex, Age, Specimen part
View SamplesExamination of whole genome gene expression profiles from the placentas of cloned (somatic cell nuclear transfer, SCNT) and normally produced (control) calves using RNA-seq.The differentially expressed genes were analyzed between SCNT and control placentas.
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Sex
View SamplesNonresolving inflammation is correlated to carcinogenesis. Ulcerative colitis-associated colorectal cancer (UC-CRC), one of the typical carcinoma generated by inflammation that cannot be resolved properly, has been widely believed to involve a multistep process contains inflammation-dysplasia-cancer sequence. The exact molecular mechanisms underlying the step-wise development of UC-CRC is still not fully understood. Detecting the changes in gene expression profiles may help to reveal why and how does the prolonged inflammatory response lead to carcinogenesis, and to characterize potential diagnostic/prognostic markers or additional therapeutic targets for UC-CRC. There for, we performed temporal genome expression profiling analysis using the Affymetrix genome wide microarray system to identify broad scale changes in gene expression associated with the development of colitis-associated cancer, based on an AOM/DSS induced mouse model of UC-CRC.
Dynamic activation of the key pathways: linking colitis to colorectal cancer in a mouse model.
Disease, Disease stage
View SamplesDamage-associated molecular pattern (DAMP) molecules S100A8 and S100A9 with well-known functions in inflammation, tumor growth and metastasis. It has been found to have promote tumor cell proliferation activity at low concentration . However, the mechanism underlying this remains unclear. In the current study, we performed genome expression profiling analysis using the Affymetrix genome wide microarray system to identify broad scale changes in gene expression associated with S100a8 or S100a9 recombinant protein stimulation in murine colon carcinoma cell line CT26.WT.
Inflammation-induced S100A8 activates Id3 and promotes colorectal tumorigenesis.
Cell line
View SamplesTo investigated a detailed analysis of the transcriptional response between epithelial Caco-2 cells with different Bifidobacterium animals subsp. lactis KLDS 2.0603 cells ( Control and cells treated by digestive tract fluids simulated ).
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View SamplesBackground: Plants, involved in highly complex and well-coordinated system, have evolved a considerable degree of developmental plasticity, thus minimize the damages caused by stresses. MicroRNAs (miRNAs) have recently emerged as key regulators in gene regulation, developmental processes and stress tolerance in plants. Results: In this study, systematic discovery of soybean miRNAs associated with stress response (mock, drought, salinity and alkalinity) has been identified and analyzed in combination with deep sequencing technology and in-depth bioinformatics analysis. We found that a total of 133 conserved miRNAs corresponding to 95 miRNA families have expressed in soybean under four diverse treatments. In addition, 71, 50 and 45 miRNAs are either uniquely or differently expressed under drought, salinity and alkalinity respectively, suggesting that many miRNAs are inducible and are differentially expressed in response to certain stress. Conclusion: Our study has important implications for further identification of gene regulation under abiotic stresses and also significantly contributes a complete profile of miRNAs in Glycine max.
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View SamplesThis study performed a transcriptomic analysis of gene expression during hair cell regeneration in the cochleae of chick after antibiotic injury in vivo.
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Sex, Specimen part, Cell line
View SamplesWe show that key regulators of oxidative metabolism, the Estrogen Related Receptors (ERRs) and the PGC-1 co-activators, are transiently induced during somatic cell reprogramming. Bioenergetic assays reveal that while glycolysis increases throughout the reprogramming transition, the early stages feature a transient oxidative phosphorylation (OXPHOS) burst. Up-regulation of ERRa or ? is a prerequisite for the OXPHOS burst in human and mouse cells, respectively, and failure to induce this metabolic switch collapses the reprogramming process. We identify a Sca1-/CD34- sub-population of early reprogramming cells with enhanced ERR? and PGC-1ß expression as bona fide reprogramming progenitors. Transcriptional profiling confirmed that these progenitors have undergone extensive metabolic reprogramming. These studies characterize a previously unrecognized, ERR-dependent metabolic switch prior to establishment of induced pluripotency.
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View SamplesHuman pancreatic adenocarcinoma cell line
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View SamplesWe show that fat-resident regulatory T cells, termed fTregs, drive age-associated insulin resistance and can be specifically depleted to increase adipose insulin sensitivity. Comparative AdipoImmune profiling in young, aged, and obese mice reveals that fTregs progressively enrich in adipose as a function of age, but not obesity. fTreg-deficient mice are protected from age-associated insulin resistance and its accompanying physiological hallmarks. In contrast, fTreg-deficiency offers no protection from obesity-associated insulin resistance.
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View Samples