Transcriptomic analysis of BnLATE-regulated pathways to investigate the genome-wide effects of BnLATE overexpression on transcription
No associated publication
None
Specimen part
View SamplesIllumina/Solexa sequencing of CG3985 mutant and wildtype testes transcriptome (0-1 day males)
A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several Y-linked male fertility genes.
None
No sample metadata fields
View SamplesThe placenta has shown morphological and functional adaption in Meishan and Yorkshire pig breeds during late gestation. While the vast difference of uterine capacity and conceptus genotype between Western and Chinese pig breeds affects the pattern of placental development. Whether the placenta within Chinese pig breed also has similar adaptive changes is still unknown. So, we measured the weight and area of 80 Chinese indigenous Diannan small-ear pig placentas and further selected 6 placentas from extremely high and low litter size groups (HL and LL) for deep RNA-sequencing to detect the molecular basis relate to the observed placental phenotype difference.
No associated publication
None
Sex, Specimen part
View SamplesThis study is aimed to compare the gene expression between wild type and 35S:6MYC-HARP1 in response to wounding treatment.
No associated publication
None
Age, Specimen part, Treatment
View SamplesTransposons in maize may be involved in the formation of circRNAs and further modulate phenotypic variation. To test our hypothesis, we performed circRNA-Seq(RNase R treated) on B73 seedlings(third leaves of V3 stage), and uncovered 1,572 high-confidence maize circRNAs, which show distinct genomic features compared to linear transcripts. Comprehensive analyses showed that LINE1-like elements (LLE) and their reverse complementary pairs (RCPLLEs) are significantly enriched in the flanking regions of circRNAs.
Circular RNAs mediated by transposons are associated with transcriptomic and phenotypic variation in maize.
None
Specimen part, Disease
View Samples1. To identify lncRNA regulating colorectal tumorigenesis, we performed RNA-seq analysis of cells with high and low tumorigenicity 2. To study the role of UPAT in colorectal cancer cells, we investigated the gene expression profiles of HCT116 cells in which UPAT expression had been suppressed by siRNA.
No associated publication
None
Cell line
View SamplesThis study provide a dynamic atlas of endosperm development from Shaanxi within China
No associated publication
None
Specimen part
View SamplesSingle cell RNA-seq was applied for studying the transcriptomic profile in early zebrafish PGCs(primordial germ cells) by choosing three time points during zebrafish embryonic development. The three time points were 6hpf(hours post fertilization, also called shield stage), 11hpf(also called 3-somite stage) and 24hpf(also called prim-5 stage).
No associated publication
None
Sex, Specimen part, Cell line
View SamplesThis study presented the differentially expressed genes post maize infected by Rhizoctonia solani.
No associated publication
None
Specimen part
View SamplesTo investigate the molecular mechanism which caused the defect of aberrant somatic cell differentiation in Wt1-deficient gonads, the differentially expressed genes between control and Wt1-deficient gonads at E11.5 was analyzed by RNA Seq analysis.After tamoxifen injection at E9.5, total RNA was prepared from control and Wt1-deficient gonads at E11.5 using RNeasy Minikit (Ambion, Austin, TX). Gonads of the same genotype (about 12 pairs each) were pooled together. For RNA-Seq, the main reagents and instruments used for RNA library construction and deep sequencing were the Illumina Gene Expression Sample Prep Kit, Solexa Sequencing Chip (flowcell), Illumina Cluster Station and Illumina HiSeq 2000 System. Sequence tags were prepared using the Illumina Digital Gene Expression Tag Profiling Kit, according to the manufacturer's protocol. Raw data was filtered to remove adaptor tags, low quality tags, and tags with a single copy number. Paired-end reads were mapped to the mouse genome (mm9) with TopHat and further analyzed by Cufflinks-Cuffdiff (Trapnell et al., 2009; Trapnell et al., 2010) pipelines to identify differentially expressed transcripts (DETs) between control and Wt1-deficient gonads.
No associated publication
None
No sample metadata fields
View Samples