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Oryza sativa
12 Downloadable Samples
Affymetrix Rice Genome Array (rice)
Description
During the pathogenesis of virus disease, lots of changes occur in plant hosts including the altered gene expression profiles. Rice stripe virus (RSV) is the type member of Tenuivirus, transmitted by Laodelphax striatellus in a circulative propagative man
Publication Title
No associated publication
Alternate Accession IDs
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 4 Downloadable Samples
Illumina HiSeq 2500
Description
Aims to find out differential expression genes (DEGs)in RAW264.7 cells during infection by Burkholderia pseudomallei infection
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Arabidopsis thaliana
- 6 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
To identify the target genes regulated by ANAC069, the expression profiles of OE (Overexpression of ANAC069) plants and KO (Knockout of ANAC069, SALK_095231C) plants under salt stress conditions were compared using Affymetrix Arabidopsis gene chips.
Publication Title
No associated publication
Alternate Accession IDs
Sample Metadata Fields
No sample metadata fields
View Samples
SRP115379- Zea mays
- 6 Downloadable Samples
- Illumina HiSeq 2000
Description
We used isolated zygotene meiocytes, corresponding anthers and 2-week-old seedlings from the Zea mays inbred line Mo17 for RNA extraction and library construction for sequencing with Illumina technology to gain insight on gene expression during a key step in meiosis when recombination initiates.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Age, Specimen part
View Samples- Citrus sinensis
- 10 Downloadable Samples
- Affymetrix Citrus Genome Array (citrus)
Description
Huanglongbing (HLB) (=citrus greening) is a destructive disease of citrus which is caused by a fastidious, phloem-inhabiting bacterium of the genus Candidatus Liberibacter. Large-scale analysis of gene expression changes in Valencia orange leaves were studied during the course of 19 weeks after inoculation with Ca. L. asiaticus using the Affymetrix GeneChip citrus genome array to provide new insights into the molecular basis of citrus response to this pathogen. Of the more than 33,000 probe sets on the microarray 21,067 were expressed in the leaves, of which 279 and 515 were differentially expressed (FDR 0.05) five to nine and 13-17 weeks after inoculation, respectively. Results from semi-quantitative RT-PCR analysis performed on 14 selected genes were highly correlated with those observed with the microarray. Gene expression changes involved a variety of different processes including cell defense, transport, cellular organization, photosynthesis, and carbohydrate metabolism. Notable was the pathogen-induced accumulation of transcripts for a phloem-specific lectin PP2-like protein. Transcriptional changes and their relation to disease symptom development are discussed. This is the first study of transcriptional profiling in citrus in response to liberibacter infection using microarray technology.
Publication Title
No associated publication
Alternate Accession IDs
Sample Metadata Fields
Specimen part
View Samples- Gallus gallus
- 6 Downloadable Samples
- Affymetrix Chicken Genome Array (chicken)
Description
Infectious bursal disease virus (IBDV) causes a highly contagious, immunosuppressive disease in chickens. The virus mainly infects immature B lymphocytes in the bursa of Fabricius (BF). Chicken B cell line DT40, an avian leukosis virus-induced B cell line, supports very virulent IBDV (vvIBDV) infection in vitro and thereby serves as a good model for investigating the infection and pathogenesis of this virus. However, a transcriptome-wide understanding of the interaction between vvIBDV and B cells has not yet been achieved. This study aimed to employ time-course DNA microarrays to investigate gene expression patterns in DT40 cells after infection with vvIBDV strain LX.
Publication Title
No associated publication
Alternate Accession IDs
Sample Metadata Fields
Specimen part, Cell line, Time
View Samples- Xenopus laevis
- 27 Downloadable Samples
- Affymetrix Xenopus laevis Genome 2.0 Array (xlaevis2)
Description
The Notch signaling pathway functions in a number of processes during embryologic development, especially the maintenance or aquisition of cell fate. We purturb the Notch signalling pathway in embryonic Xenopus laevis in order to 1) better characterize the downstream targets of Notch signalling, and 2) determine the extent to which early embryos can recover from transient purturbations to critical signalling pathways, if at all.
Publication Title
No associated publication
Alternate Accession IDs
Sample Metadata Fields
No sample metadata fields
View Samples- Arabidopsis thaliana
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
It has been reported that IRE1-bZIP60 pathway is conserved for the signal transduction and gene regulation in response to ER stress including high temperature among human, yeast, Arabidopsis, rice and maize. However, the information of their counterparts in wheat is limited and, especially, the biological relevance of the equivalent pathway contributing to heat tolerance is still ambiguous. In present study, we identified heat stress-induced wheat bZIP60 and investigated its splicing pattern depending on IRE1.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Specimen part, Treatment
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 ?C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 ?C under dark conditions, the immature embryos were collected, and stored at -70 ?C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Specimen part
View Samples