NRVMs were subjected to varying durations of ischemia or ischemia+reperfusion using coverslip hypoxia. Expression profiling was used to identify genes that are differentially regulated in either event.
No associated publication
No sample metadata fields
View SamplesFetal, perinatal and adult mouse mammary epithelial single cell transcriptomes
No associated publication
None
Sex, Specimen part, Disease, Cell line
View SamplesThese RNA-seq data are from dorsolateral prefrontal cortex (DLPFC) tissue from 5 individuals left off ice at room temperature for 0, 15, 30, and 60 minutes. Resulting RNA was extracted and sequenced with both polyA+ and RiboZero protocols.
No associated publication
None
Sex, Age, Specimen part, Disease, Race
View SamplesThis study was designed to understand the mechanism by which floral organ abscission mutants'' phenotypes arise.
No associated publication
None
Specimen part, Treatment
View SamplesGlobal restriction of protein synthesis is a hallmark of cellular stress. Using hydrogen peroxide, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to separate highly translated and poorly translated mRNAs that were then separately analysed.
Global translational responses to oxidative stress impact upon multiple levels of protein synthesis.
None
Sex, Compound
View SamplesOne common form of translational control is mediated by proteins that bind to the mRNA 5' cap-binding protein eIF4E. These proteins are collectively called 4E binding proteins (4EBPs). Saccharomyces cerevisiae possesses two 4EBPs that are encoded by non-essential genes called CAF20 and EAP1. To determine the impact of gene deletion on gene expression, we monitored the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes in wild-type, caf20 and eap1 cells. Polyribosome fractionation of cell extracts was used to separate highly translated and poorly translated mRNAs that were then separately analyzed.
Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins.
None
Sex
View SamplesNeuro-2a cells were infected with ZIKV (MOI = 0.5) for 48 h and total RNA was extracted and purified using TRI Reagent and RNeasy Mini kit (Qiagen). RNA-seq was performed at the Molecular and Genomics Core Facility of the University of Mississippi Medical Center. Differential expression analysis between mock and ZIKV infected cells was done using Tophat and cuffdiff programs from the tuxedo suite.
No associated publication
None
Sex, Specimen part, Disease, Cell line
View SamplesEffect of FGF2 on the transcriptional profile of microvascular endothelial cells
A pro-inflammatory signature mediates FGF2-induced angiogenesis.
None
Specimen part, Cell line, Compound
View SamplesCompare m1A levels in the 16S (large) mitochondrial ribosomal RNA in TRMT61B knockdown cells and control.
No associated publication
None
Sex, Specimen part
View SamplesRNA-seq analysis of nco (no content) zebrafish embryos at 24 hours post fertilization.
No associated publication
None
Sex, Specimen part, Cell line
View Samples