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GSE2515
Mus musculus
6 Downloadable Samples
Affymetrix Mouse Expression 430A Array (moe430a)
Description
TEC (B6 vs Aire KO). Thymic epithelial cells from B6 strain and AIRE KO are compared.
Publication Title
No associated publication
Alternate Accession IDs
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 6 Downloadable Samples
IlluminaHiSeq1000
Description
Superior frontal gyrus grey and white matter
Publication Title
Unique transcriptome patterns of the white and grey matter corroborate structural and functional heterogeneity in the human frontal lobe.
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Arabidopsis thaliana
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
to compare the transcriptome between WT and skl1 mutant
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 5 Downloadable Samples
- Illumina HiSeq 2500
Description
The Mechanisms of Maize Resistance to Fusarium verticillioides by comprehensive analysis of RNA-seq Data
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Age, Specimen part, Disease, Disease stage, Treatment
View Samples- Danio rerio
- 5 Downloadable Samples
- IlluminaHiSeq2500
Description
A genome-wide map of circular RNA in adult zebrafish.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
Spermatogenesis is a complex differentiation process that involves the successive and simultaneous execution of three different gene expression programs: mitotic proliferation of spermatogonia, meiosis, and spermiogenesis. Testicular cell heterogeneity has hindered its molecular analyses. Moreover, the characterization of short, poorly represented cell stages such as initial meiotic prophase ones (leptotene and zygotene) has remained elusive, despite their crucial importance for understanding the fundamentals of meiosis.We have developed a flow cytometry-based approach for obtaining highly pure stage-specific spermatogenic cell populations, including early meiotic prophase. Here we combined this methodology with next generation sequencing, which enabled the analysis of meiotic and postmeiotic gene expression signatures in mouse with unprecedented reliability. Interestingly, we found that a considerable number of genes involved in early as well as late meiotic processes are already on at early meiotic prophase, with a high proportion of them being expressed only for the short time lapse of lepto-zygotene stages. Besides, we observed a massive change in gene expressionpatterns during medium meiotic prophase (pachytene) when mostly genes related to spermiogenesis and sperm function are already turned on. This indicates that the transcriptional switch from meiosis to post-meiosis takes place very early, during meiotic prophase, thus disclosing a higher incidence of post-transcriptional regulationin spermatogenesis than previously reported. Moreover, we found that a good proportion of the differential gene expression in spermiogenesis corresponds to up-regulation of genes whose expression starts earlier, at pachytene stage; this includes transition protein- and protamine-coding genes, which have long been claimed to switch on during spermiogenesis. In addition, our results afford new insights concerning X chromosome meiotic inactivation and reactivation. This work provides for the first time an overview of the time course for the massive onset and turning off of the meiotic and spermiogenic genetic programs. Importantly, our data represent a highly reliable information set about gene expression in pure testicular cell populations including early meiotic prophase, for further data mining towards the elucidation of the molecular bases of male reproduction in mammals.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Homo sapiens
- 2 Downloadable Samples
- Illumina HiSeq 4000
Description
Transcriptomic sequencing of human gastric cancer cell line (AGS) upon citral treatment
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line
View Samples- Homo sapiens
- 1 Downloadable Sample
Description
We propose that the stochastic combination of alternative transcription events has contributed to complex isoform evolution.
Publication Title
Evolutionary interrogation of human biology in well-annotated genomic framework of rhesus macaque.
Alternate Accession IDs
None
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 3 Downloadable Samples
- Illumina HiSeq 4000
Description
Cervical cancer cell line C33A
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment, Race
View Samples- Mus musculus
- 3 Downloadable Samples
- NextSeq 500
Description
In the terms of vaccine efficacy and duration of protection in malaria vaccination is major concern against malaria. On the other hand, it is facing complications in development and administration to the host. However, whole sporozoites vaccination (WSV) is far more efficacious than any other alternative strategy. We have found that the intermittent sporozoite challenge to immune mice following RAS vaccination extends the longevity of sterile protection by maintaining CD8+T cell memory responses to LS infection and also helps in CD8a+DCs accumulation and activation in liver. Consequently, there has been great interest in elucidating and understating the sterile immunological response at mechanistic level. The information we have generated can then potentially be used in generation of next generation vaccine with improved efficacy and duration of protection. In this work, to elucidate the host initial immune response underlying the protective effects of a WSV in shaping the protected sterile protection and advances its immunogenicity in the future, a high-throughput RNA sequencing technology was used to investigate the immunization related gene expression patterns of mouse immunized with radiation attenuated sporozoites (RAS) vaccine.
Publication Title
No associated publication
Alternate Accession IDs
None
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples