This project aims at an initial characterization of changes in gene expression in zebrafish with advancing age. Transcript levels are determined in several tissues of zebrafish with differing ages using RNA-seq. Differentially expressed genes are determined to pinpoint genes that are differently regulated in young and old zebrafish. Results will be compared with other species to identify common pathways of ageing.
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View SamplesWe analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.
Gene expression signature in organized and growth-arrested mammary acini predicts good outcome in breast cancer.
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Sex, Specimen part, Cell line, Time
View SamplesTranscriptome of human HL-60 and HEK-293 cells depending on culture cell density
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View SamplesCharacterization of circular RNAs in Ovarian cancer
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View SamplesTo investigate the effect of local genetic variation on heritable traits, we developed a new recombinant inbred line (RIL) population derived from four wild isolates. The wild isolates were collected from two closely located sites in France: Orsay and Santeuil. By crossing these four genetically diverse parental isolates a population of 200 RILs was constructed. RNA-seq was used to obtain sequence polymorphisms
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Sex, Specimen part, Cell line
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Age, Specimen part
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Sex, Age, Specimen part, Disease
View SamplesIn mice, zygotic activation mainly occurs on a wide variety of genes mainly at the 2-cell stage. Long noncoding RNAs (lncRNAs) are increasingly being recognized as molecules that modulate gene expression. In this study, we performed the directional RNA-seq of MII oocytes and 2-cell embryos to identify the long noncoding RNAs activated during ZGA.
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View SamplesVHL inactivation is a major component of renal cancer. In this study we re-engineered RCC4 cells to re-express VHL and performed mRNA-sequencing on triplicates to analyse gene and transcript expression changes specific to VHL inactivation.
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View SamplesWe first offered a comprehensive transcriptome from three pig adipose tissues (Hypodermal layer of backfat, greater omentum and mesenteric adipose tissue) by high-throughput RNA-Seq.
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Sex, Specimen part
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