we aimed to calculate the expression level of some special genes
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View SamplesWe have studied the transcriptome after REM sleep deprivation and compared to normal control.
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View SamplesFree moving control
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View SamplesREM sleep deprived rat
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View SamplesTest effects of mtDNA variation on nuclear transcript expression using various mtDNA haplotypes on isogenic nuclear backgrounds
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Sex, Age, Specimen part, Cell line
View SamplesMitonuclear transcriptomics
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Sex, Age, Specimen part, Cell line, Treatment
View SamplesThe goal of this study is to characterize the genes that are specifically expressed in the shaft cells of olfactory sensory organ precursors, and regulate nanopore formation on cuticular sheath. To this end, pupal antennae of a wild type strain and two mutant strains (amos and neur>Nb) of Drosophila were subjected to RNA-seq analysis.
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Sex, Specimen part, Cell line
View SamplesMicroarray technologies allow the identification of large numbers of expression differences within and between species. Although environmental and physiological stimuli are clearly responsible for changes in the expression levels of many genes, it is not known whether the majority of changes of gene expression fixed during evolution between species and between various tissues within a species are caused by Darwinian selection or by stochastic processes. We find the following: (1) expression differences between species accumulate approximately linearly with time; (2) gene expression variation among individuals within a species correlates positively with expression divergence between species; (3) rates of expression divergence between species do not differ significantly between intact genes and expressed pseudogenes; (4) expression differences between brain regions within a species have accumulated approximately linearly with time since these regions emerged during evolution. These results suggest that the majority of expression differences observed between species are selectively neutral or nearly neutral and likely to be of little or no functional significance. Therefore, the identification of gene expression differences between species fixed by selection should be based on null hypotheses assuming functional neutrality. Furthermore, it may be possible to apply a molecular clock based on expression differences to infer the evolutionary history of tissues.
A neutral model of transcriptome evolution.
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Sex, Age, Specimen part, Disease, Disease stage
View SamplesIn present, interspecies cloning and interspecies-pregnancy were studied for endangered species rescue. However, the low implantation and survival ratio, spontaneous abortion, and unknown reason embryos absorption are the common and difficult problems of interspecies-pregnancy. In order to discover the mechanism of interspecies-pregnant failure and find ways to overcome the xeon-pregnant obstacles, we chosen the rat embryos pregnant in mouse uterus as a interspecies-pregnancy model. Three groups were set, mouse embryos to mouse recipients (MM) as control group, rat embryos to mouse recipients (RM), and rat and mouse embryos to mouse recipients together (RMM) as experiment groups. The former studies showed that rat embryos live no longer than day 7 of mouse pregnancy (D7). Our results showed that rat embryos survived to D7, and still existed to day 9 of mouse pregnancy (D9) in RM group. Surprisingly, the rat embryos survived to day 13 of the mouse gestation (D13) in RMM group. Microarray analysis was used to detect the global-gene expression profile changes of the whole implantation sites among the three groups at D7 and D9. By this way, we screened out the genes promoting the implanted rat embryos development in a mouse uterus which helped the rat embryos survive to D13 in RMM group compared with RM group, and the genes hindering the rat embryos development in a mouse uterus which prevented rat embryos living longer than D7 in RM group and D13 in RMM group compared with MM group. These findings provide insights into the mechanism of interspecies pregnant failure and new idea for interspecies pregnant studies.
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Sex, Specimen part, Treatment
View SamplesExpression data from Caenorhabditis elegans let-418(RNAi), mep-1(RNAi) and gfp(RNAi) L1 larvae.
Different Mi-2 complexes for various developmental functions in Caenorhabditis elegans.
Disease
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