We generated the induced salivary gland rudiment (iSG) from mouse embryonic stem cells (ESCs). To characterize the iSG, we compared the gene expression profiles of the iSG, which were dissected from the protruded region of ESC-derived aggregates with those of the embryonic submandibular gland in each developmental stage via RNA-seq.
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Specimen part, Cell line
View SamplesRNA-sequencing analysis of RBP2 overexpressing MCF7 cell lines. RBP2 (also known as JARID1A), a member of the JARID1 family of histone H3 lysine K4 demethylases, has been considered to have an oncogenic potential in several cancer including breast cancer. Results provide insight into the transcriptional regulation of RBP2 in estrogen receptor positve breast cancer.
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Sex, Age, Specimen part, Disease, Cell line
View SamplesHLA-I positive and negative subpopulations isolated from human clear cell sarcoma xenografts
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Sex, Specimen part, Disease
View SamplesDysfunction of Th17 cells leads to chronic inflammatory disorders. STAT3 orchestrates the expression of proinflammatory cytokinesand pathogenic cell differentiation from IL-17-producing Th17 cells. However, the pathways mediated by STAT3 signaling are not fully understood. Here, we observed that FRA1 and JUNB are directly involved in STAT3 binding to sites in the promoters of Fosl1 and Junb. Promoter binding increased expression of IL-17 and the development of Th17 cells. Overexpression of Fra1 and Junb in mice resulted in susceptibility to collagen-induced arthritis (CIA) and an increase in Th17 cell numbers and inflammatory cytokine production. In patients with rheumatoid arthritis (RA), FRA1 and JUNB were colocalized with STAT3 in the inflamed synovium. These observations suggest that FRA1 and JUNB are associated closely with STAT3 activation, and that this activation leads to Th17 cell differentiation in autoimmune diseases and inflammation.
No associated publication
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Sex, Specimen part, Cell line
View SamplesTo evaluate the mRNA profiles of the wild-type N2 and the innate immunity-deficient mutant rde-1 strains of C. elegans upon Orsay virus infection.
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Specimen part
View SamplesGlycine max and Phytophthora sojae infected Glycine max Transcriptome
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Specimen part
View SamplesThe project was aim of search the different mechanism of resoonse to soybean cyst nematode and mining the candidate resisitance genes from next generation sequencing
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Specimen part, Disease, Disease stage
View SamplesIntratumoral heterogeneity is common in all tumor types and major barrier against effective cancer therapy. Especially, human malignant mesothelioma that is an environmental contaminant- induced disease is known to be extremely heterogeneous with regards to morphology as well as molecular phenotypes, resulting in poor response to cancer therapy. This study was performed to investigate the global gene expression profile of distinct subpopulations of MS1, a human malignant mesothelioma cell line
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View SamplesThe dual leucine zipper kinase (DLK) is a mitogen-activated kinase kinase kinase that can activate the downstream cJun N terminal kinase (JNK) pathway (ref). We have previously reported that DLK is a positive regulator of the retrograde injury signaling and axon regeneration that unfolds after sciatic nerve injury (ref). Since DLK is required for activities of injury-associated transcription factors such as cJun and STAT3, we hypothesized that DLK is also necessary for the transcriptional responses to peripheral nerve injury. In the current study, we identify DLK-dependent transcriptome in dorsal root ganglion (DRG) neurons using a sciatic nerve injury paradigm. The DEG analysis reveals that DLK regulates regeneration/injury-associated genes in both basal and injured conditions. By performing gene ontology analysis, we suggest functional annotations and the involved genes as regulatory components of the axonal regeneration program. Finally, our comparative analysis indicates that DLK is required for a specific retrograde signaling pathway that regulates a regeneration program shared between PNS and CNS models.
No associated publication
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Sex, Specimen part
View Sampleswe have compared the injury-induced transcriptomic changes between the regenerating proximal segment and the degenerating distal segment of a transected nerve, at different post-injury time points
No associated publication
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Sex, Age, Specimen part
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