This study was designed to understand the mechanism by which floral organ abscission mutants'' phenotypes arise.
No associated publication
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Specimen part, Treatment
View SamplesNeuro-2a cells were infected with ZIKV (MOI = 0.5) for 48 h and total RNA was extracted and purified using TRI Reagent and RNeasy Mini kit (Qiagen). RNA-seq was performed at the Molecular and Genomics Core Facility of the University of Mississippi Medical Center. Differential expression analysis between mock and ZIKV infected cells was done using Tophat and cuffdiff programs from the tuxedo suite.
No associated publication
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Sex, Specimen part, Disease, Cell line
View SamplesRNAseq from male testes
Odorant receptor-mediated sperm activation in disease vector mosquitoes.
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Sex, Specimen part
View SamplesThe dual leucine zipper kinase (DLK) is a mitogen-activated kinase kinase kinase that can activate the downstream cJun N terminal kinase (JNK) pathway (ref). We have previously reported that DLK is a positive regulator of the retrograde injury signaling and axon regeneration that unfolds after sciatic nerve injury (ref). Since DLK is required for activities of injury-associated transcription factors such as cJun and STAT3, we hypothesized that DLK is also necessary for the transcriptional responses to peripheral nerve injury. In the current study, we identify DLK-dependent transcriptome in dorsal root ganglion (DRG) neurons using a sciatic nerve injury paradigm. The DEG analysis reveals that DLK regulates regeneration/injury-associated genes in both basal and injured conditions. By performing gene ontology analysis, we suggest functional annotations and the involved genes as regulatory components of the axonal regeneration program. Finally, our comparative analysis indicates that DLK is required for a specific retrograde signaling pathway that regulates a regeneration program shared between PNS and CNS models.
No associated publication
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Sex, Specimen part
View Sampleswe have compared the injury-induced transcriptomic changes between the regenerating proximal segment and the degenerating distal segment of a transected nerve, at different post-injury time points
No associated publication
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Sex, Age, Specimen part
View SamplesViral and bacterial coinfections are common in nature, but infrequently studied in laboratory models of infection. We observed disease severity differences in mice infected with two of three possible respiratory viruses, depending on the order of the infection. To discover the mechanisms causing these differences, we compared gene expression responses of lung tissue at three time points following viral coinfection. Differential gene expression and immune cell counts suggest a dampening of immune responses in mice infected with rhinovirus followed by influenza A virus or pneumonia virus of mice.
No associated publication
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Sex, Specimen part, Cell line, Treatment
View SamplesRNA-sequencing was used on leaf tissue from 29 diverse maize lines to characterize differences in gene expression between lines. The main goal from this study was to relate gene expression to measured traits of interest.
No associated publication
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Age, Specimen part
View SamplesC2C12 cells, as mouse-derived myoblasts, are a classic cell model for studying skeletal muscle development. We knocked down the immunoglobulin superfamily containing leucine-rich repeat (Islr) gene in C2C12 cell line and studied the effect of Islr on the proliferation and differentiation of C2C12 cells.
No associated publication
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Sex, Specimen part, Cell line
View SamplesThis project sequences the mRNA of the chorioallantoic membrane of Gallus gallus, the domestic chicken.
No associated publication
None
Sex, Specimen part, Cell line
View SamplesIt has been demonstrated previously that the reprogramming factors are sequestered in the pronuclei of zygote after fertilization, as the enucleated zygotes at interphase cannot support the development of cloned embryos whereas the enucleated zygotes at M-phase can reprogram somatic cells to full pluripotency. However, it remains unknown whether the parental pronucleus, derived either from the sperm or oocyte, possesses the similar reprogramming ability. Here, we provide evidence demonstrating that the parental pronuclei are asymmetric in reprogramming and the reprogramming factors reside mainly in the male pronucleus. As a result, only the female pronucleus-depleted mouse zygotes enucleated at M-phase of mitosis can support the somatic cell reprogramming, the derivation of chromosome transfer embryonic stem (ctES) cells with full pluripotency and the full term development of cloned embryos. In striking contrast, the male pronucleus-depleted zygotes enucleated at M-phase of mitosis fail to support the pre-implantation development of somatic cell cloned embryos. Furthermore, we demonstrated that the distinct epigenetic reprogramming ability of the parental pronucleus might contribute directly to the developmental difference of somatic cloned embryos. Our study highlights the developmental asymmetry of parental pronuclei in reprogramming.
Asymmetric reprogramming capacity of parental pronuclei in mouse zygotes.
Cell line
View Samples