Transcripomic analysis of leaf gene expression in S and N-deficient winter wheat during grain development. Tissue was harvested at anthesis and 7, 14 and 21 days post anthesis from experimental field plots.
Co-ordinated expression of amino acid metabolism in response to N and S deficiency during wheat grain filling.
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Specimen part, Disease, Disease stage, Subject, Time
View SamplesPseudomonas aeruginosa transcriptomic profile exposed to Cr(VI)
No associated publication
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Specimen part, Disease
View SamplesGlycine max and Phytophthora sojae infected Glycine max Transcriptome
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Specimen part
View SamplesThe project was aim of search the different mechanism of resoonse to soybean cyst nematode and mining the candidate resisitance genes from next generation sequencing
No associated publication
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Specimen part, Disease, Disease stage
View SamplesIn this study, we performed a NGS analysis to identify transcriptome landscape of the human placenta during uncomplicated single and twin pregnancy, to establish a pattern of normal placental genes expression for further comprehensive analyses. Additionall aim of these studies was to identify the differentially expressed transcripts of genes in single and twin pregnancy that may participate in human pregnancy.
No associated publication
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Sex, Age, Specimen part, Disease
View SamplesMutations mapping to the RNA-binding interface of EXOSC3 have been linked to the rare neurological disorder known as Pontocerebellar Hypoplasia type1B (PCH1B). EXOSC3 is part of three putative RNA-binding structural cap proteinsthat guideRNA intothe RNA exosome, thecellular machinery that2degrades RNA. Here, using RNAcompete, we identifieda G-rich RNA motifthat requirestheK homology and ribosomal protein S1domains of EXOSC3. Interestingly, several PCH1B-causing mutations in EXOSC3do not engage this RNA motif. To test the hypothesis thatmodificationof the RNA-protein interface in EXOSC3 mutants may be phenocopied by small molecules, we performedan in silicoscreen of 50,000 small molecules and used enzyme-linked immunosorbant assays(ELISAs)to assess the ability ofthe molecules to inhibit RNA-bindingbyEXOSC3. We identified asmall molecule, EXOSC3-RNA disrupting (ERD) compound 3 (ERD03), which: (i) bound specifically to EXOSC3in saturation transfer difference nuclear magnetic resonance (STD NMR); (ii)disruptedthe EXOSC3-RNAinteraction in a concentration-dependent manner; (iii) induced an abnormal curved spine PCH1B-like phenotype in zebrafish embryos.This compound induced a comparable modification of RNA expression levels coupled with an atrophy of the cerebellum in the zebrafish. To our knowledge, this is the first example of a small molecule obtained by rational design thatmodelsthe abnormal developmental effectsof a neurodegenerative diseasein a whole organism.
No associated publication
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Sex, Age, Specimen part, Disease, Treatment
View SamplesViral and bacterial coinfections are common in nature, but infrequently studied in laboratory models of infection. We observed disease severity differences in mice infected with two of three possible respiratory viruses, depending on the order of the infection. To discover the mechanisms causing these differences, we compared gene expression responses of lung tissue at three time points following viral coinfection. Differential gene expression and immune cell counts suggest a dampening of immune responses in mice infected with rhinovirus followed by influenza A virus or pneumonia virus of mice.
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Sex, Specimen part, Cell line, Treatment
View SamplesArabidopsis thaliana Transcriptome (Vv-circATS1-OE and WT under 4?)
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Specimen part
View SamplesTo obtain more insight into the molecular changes associated with PPD down-regulation, RNA was extracted from the first leaf pair of ami-ppd and wild-type plants at 13 DAS (the time point at which differences in leaf area start to be visible), and subjected to micro-array transcript profiling.
No associated publication
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Specimen part, Time
View SamplesWild type and transgenic msi1-tap1 plants were grown and gene expression was compared at two time points at the age of 8 days.
Regulation of flowering time by Arabidopsis MSI1.
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Age, Time
View Samples