The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from Leanworthia crassa (Lcr) to complement an Arabidopsis LFY mutant. Plants used are homozygous lfy6 mutants (EMS alleles) in Ler background which are transformed or not (for the lfy6 mutant) by genomic clones for Arabidopsis LFY (AthLFY) or Leanworthia crassa LFY (LcrLFY). Flowering was synchronized by growing plants in SD then shifting them to LD. 2 time points samples (wild type Ler) were taken at the end of the SD period as a reference for genes induced by shifting to LD, irrespective of the status at the LFY locus.
Evolutionary divergence of LFY function in the mustards Arabidopsis thaliana and Leavenworthia crassa.
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Age, Specimen part, Time
View SamplesSeven-day-old white-light-grown Arabidopsis seedlings were exposed for 15 minutes to polychromatic radiation with decreasing short-wave cut-off in the UV range, transferred back to the standard growth chamber and samples were taken 1 and 6 hours after the start of irradiation.
Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis.
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Age, Time
View SamplesWT cells and mutants during growth on low phosphate levels and recovery
No associated publication
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Specimen part, Disease, Cell line
View SamplesWT and mutants cells during growth in low phosphate levels and recovery into 20mM phosphate
No associated publication
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Specimen part, Disease, Cell line
View SamplesBased on the pkl dP mutant phenotype, it is predicted that PKL plays a key role in regulating GA-responsive genes that are important for vegetative growth and phase transitions. To test this hypothesis, global transcriptome analysis was performed by RNA-Seq using shoots of 13d-old ga1-13 and ga1-13 pkl that were mock-treated or 10 µM GA3-treated for 24 h.
No associated publication
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Age, Specimen part
View SamplesTissues of Arabidopsis plants overexpressing artificial microRNAs were compared to wild_type and respective target gene mutants (duplicate arrays)
Highly specific gene silencing by artificial microRNAs in Arabidopsis.
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Specimen part
View SamplesIsogenic UPF1+ or upf1- yeast strains were treated with 10 ug/ml thiolutin to inhibit global transcription. Targets were obtained from 16 time points: 0, 2, 4, 6, 8, 10, 12, 15, 20, 25, 30, 35, 40, 45, 50, 60 minutes after transcription inhibition. Three biological replicates of each were generated and the expression profiles were determined using Affymetrix YG-S98 arrays. Comparisons between the sample groups allow the identification of genes with differential expression over time between UPF1+ and upf1-.
Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast.
No sample metadata fields
View SamplesRat mammary glands were obtained from individual rats in RXR treated (a) and control (b) conditions (12 rats in each condition). The 24 samples were hybridized individually. Also, in each condition, samples were combined into different pools of 2, pools of 3, pools of 12. Technical replicates were also run.
On the utility of pooling biological samples in microarray experiments.
No sample metadata fields
View SamplesTransgenic animals were engineered to express human amyloid peptide controlled by a muscle-specific, heat-inducible promoter. At low temperatures (16C) Abeta expression is minimal, while at higher temperatures (20-25C) Abeta accummulates in large quantities and causes paralysis.
Identifying Aβ-specific pathogenic mechanisms using a nematode model of Alzheimer's disease.
Time
View SamplesProtein and mRNA levels for several selenoproteins, such as glutathione peroxidase-1 (Gpx1), are down-regulated dramatically by selenium (Se) deficiency.
Selenium toxicity but not deficient or super-nutritional selenium status vastly alters the transcriptome in rodents.
Specimen part, Treatment
View Samples