It has been reported that IRE1-bZIP60 pathway is conserved for the signal transduction and gene regulation in response to ER stress including high temperature among human, yeast, Arabidopsis, rice and maize. However, the information of their counterparts in wheat is limited and, especially, the biological relevance of the equivalent pathway contributing to heat tolerance is still ambiguous. In present study, we identified heat stress-induced wheat bZIP60 and investigated its splicing pattern depending on IRE1.
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Specimen part, Treatment
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 ?C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 ?C under dark conditions, the immature embryos were collected, and stored at -70 ?C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
None
Specimen part
View Samples