A near-isogenic rice line CSSL50-1 with high chalkiness and Asominori (the parental line) with normal grain endosperm were used for comparative studies of rice grain endosperm chalkiness,transcriptome comparison of 15 DAF caryopses using Affymetrix rice GeneChip identified differential expressed genes between these two lines.
Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice.
None
Age, Specimen part, Time
View SamplesWe have found that overexpression of OsNPR1, a master gene for SAR in rice, greatly enhanced disease resistance. However, the growth and development of the OsNPR1 overexpression (OsNPR1-OX) lines were restrained and the mechanism remained elusive.
The Systemic Acquired Resistance Regulator OsNPR1 Attenuates Growth by Repressing Auxin Signaling through Promoting IAA-Amido Synthase Expression.
Specimen part
View SamplesThe differentiation and proliferation of chicken B cells rely on the antigens on the cell surface of bursal stromal cells. Chicken stem cell antigen 2 (SCA2) is localized on the surface of bursal cortical and medullary epithelial cells. The signals through SCA2 may regulate the expressions critical for the B cell development.
Cloning and functional characterization of chicken stem cell antigen 2.
Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Identification of artifactual microarray probe signals constantly present in multiple sample types.
Specimen part
View SamplesThe correlation of the RNA profiles obtained by microarray analysis was compared with that obtained from RNA-Seq by using reduced complexity sperm datasets. This resolved as a series of discordant probes. The extent of discordancy among other datasets was then determined.
Identification of artifactual microarray probe signals constantly present in multiple sample types.
Specimen part
View SamplesRNA-sequencing was used to screen all differentially expressed (DE) lncRNAs and mRNAs between the RSD and control groups. In mice experiencing RSD, 373 and 454 lncRNAs, along with 1142 and 654 mRNAs were significantly upregulated and downregulated, respectively.Available informatics evidence highlighted the likely role of synapse dysfunction and abnormal synaptic neurotransmission in these behaviors. Thus, our findings provide potential candidate biomarkers or intervention targets for chronic psychological stress-induced neuropsychiatric disorders. Overall design: Total RNA obtained from prefrontal cortex of mice subjected to repeated social defeat stress compared to untreated control mice.
RNA-Sequencing and Bioinformatics Analysis of Long Noncoding RNAs and mRNAs in the Prefrontal Cortex of Mice Following Repeated Social Defeat Stress.
Specimen part, Cell line, Subject
View SamplessiRNA-mediated knockdown of MIF expression in HEK293 cells resulted in inhibition of cell proliferation and cell cycle progress. The microarray study of MIF KD cells reveald knockdown of MIF would lead to extensive changes in gene expression profiles which may elucidate the molecular mechanism of MIF siRNA-mediated inhibition of cell cycle and cell proliferation.
A global genomic view of MIF knockdown-mediated cell cycle arrest.
Cell line
View SamplesThe correlation of the RNA profiles obtained by microarray analysis was compared with that obtained from RNA-Seq by using reduced complexity sperm datasets. This resolved as a series of discordant probes. The extent of discordancy among other datasets was then determined. Overall design: A correlative study between probe’s signal intensity from Illumina bead arrays with its transcript level detected by next generation sequencing technique was performed. RNAs from sperm and testis samples were applied
Identification of artifactual microarray probe signals constantly present in multiple sample types.
Specimen part, Subject
View SamplesWe deleted Tfr1 in the heart to determine the role of Tfr1 in iron uptake in normal cardiac funciton We used microarrays to identify global gene changes associated with deletion of Tfr1 in skeletal muscle
Lethal Cardiomyopathy in Mice Lacking Transferrin Receptor in the Heart.
Age, Specimen part
View SamplesRNA-Seq technique was applied to investigate the effects of four cDNA amplification kits and two RNA-Seq library preparation kits to the deep sequencing results at different perspectives. Overall design: The same set of semen samples were applied to investigate the qualitative and quantitative effect of four cDNA amplification methods and two RNA-Seq library preparation methods on sperm transcript profiling.
A comparison of sperm RNA-seq methods.
No sample metadata fields
View Samples