poly-A mRNA was purified from 4g of total RNA, fragmented and randomly primed for reverse transcription to generate double stranded cDNA. The cDNA fragments were then subjected to an end repair process, consisting of the addition of a single A base, and the ligation of indexed Illumina adapters at both ends of cDNA.

Illumina HiSeq 2500 paired end sequencing; RNAseq of Capra hircus: mucosa and lymph node infected by Haemonchus contortus and controls

Capra hircus

We compare transcriptome profiling of abomasal mucosa and lymph node tissues from non-infected, resistant and susceptible infected Creole goats using RNA-sequencing.

Transcriptome variation in response to gastrointestinal nematode infection in goats

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