Microbiota-driven regulation in small intestinal epithelial cells [RNA-seq]

We compare transcriptomic profiles of intestinal epithelial cells obtained from the small intestine of germ-free and conventionally-caged mice. Intestinal epithelial cells were harvested from the intestine of conventional or germ-free C57Bl6J mice. Directional polyA RNA-seq was performed on RNA fom cells using standard Illumina protocols. Microbiota induce decreased expression of the Clec2e gene. Overall design: Intestinal epithelial cells were harvested from the intestine of conventional or germ-free C57Bl6J mice.

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Woo V, Eshleman EM, Rice T, Whitt J, Vallance BA, Alenghat T