Transcriptome and differential gene expression analysis for nacRNAi and non-transgenic sibling

We report the transcriptome profile of the developing maize endosperm of nacRNAi. The nacRNAi is the transgenic maize which knocks down the expression of both ZmNAC128 and ZmNAC130 in the developing endosperm.ZmNAC128 and ZmNAC130 are the two maize endosperm-specific NAC-type transcription factors. This study finds that nacRNAi has a broad effect on the accumulation of starch and protein by regulating their main synthetic genes. Overall design: The 20-DAP endosperm of wild type (B73) and nacRNAi siblings (Ni4) are used to do RNA-seq. There are three replicats per line, total six samples.

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Zhang Z, Dong J, Ji C, Wu Y, Messing J