Quantitative analysis of transcriptome of wild-type and mcd1-1 mutant of Saccharomyces cerevisiae by PolyA RNAseq

In this study, we performed RNAseq of PolyA enriched RNA from budding yeast cells that were either wild-type or having the mcd1-1 mutation. By obtaining over 200 million reads of sequences from PolyA RNA, we mapped greater than 30 million reads per sample to the yeast genome (S288C R64-2-1_20150113)and generated transcriptomic profiles of wild-type and mcd1-1 mutant cells. Comparison of the wild type and mutant transcriptomes revealed that 11.44% genes were upregulated, 4.99% were downregulated and 83.57% were unaffected in the mutant with a fold change =2.0 and Padj value <0.05. When the alterations in gene expression were analyzed with respect to chromosomal position, preferential derepression of subtelomeric (located within 20 kilo bases from the chromosome end) genes was observed in the mutant. Overall design: PolyA mRNA profiles of wild type (WT) and mcd1-1 budding yeast cells were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X.

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Kothiwal D, Laloraya S