Astrocyte-to-astrocyte contact and a positive feedback loop of growth factor signaling regulate astrocyte maturation

Astrocytes were purified from postnatal day 2 mouse cortices by immunopanning with HepaCAM. Inhibitors and DMSO were added on in-vitro day 2. HBEGF was depleted on the cell prep day and till in-vitro day 7. In-vitro day 2, 7 and 14 samples were collected on designed timepointed. Overall design: Three EGFR inhibitor samples and three relative DMSO control samples, three P53 inhibitor samples and three relative DMSO control samples, two HBEGF samples and two HBEGF withdrawal samples, three samples for each in-vitro day 2, 7 and 14.

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Li J, Khankan RR, Caneda C, Godoy MI, Haney MS, Krawczyk MC, Bassik MC, Sloan SA, Zhang Y