RNA extracted using Purezol, then treated with DNase I. Samples then underwent ribsomal depletion RNA-Seq library preperation before strand-specific sequencing using an Illumina Hi-Seq 2500

Illumina HiSeq 2500 paired end sequencing; Leaf RNA-Seq of Leptochloa dubia

Disakisperma dubium Leaf RNA-Seq

Dissecting the evolutionary path to C4 photosynthesis is a critical step in engineering improvements in crop photosynthesis and yields. Three C4-acid decarboxylation enzymes (NADP-ME, NAD-ME and PEPCK) are responsible for delivering CO2 for fixation by Rubisco during C4 photosynthesis. Decarboxylase recruitment has evolved independently multiple times throughout C4 lineages. Here, a systematic yet rapid identification of key differences across species has allowed us to investigate the regulation and evolution of the three C4-acid decarboxylases in one C3 and eleven C4 grasses (Poaceae).

Transcript and amino acid variation in C4 specific decarboxylases

Submitted by Western Sydney University on 08-JAN-2019

Investigating the NAD-ME biochemical pathway within C<sub>4</sub> grasses using transcript and amino acid variation in C<sub>4</sub> photosynthetic genes.