Small RNA and degradome sequencing in Oryza species

To identify the reproductive phased secondary, small interfering RNAs (phasiRNAs) in Oryza , we performed high-throughput sRNA sequencing (sRNA-seq) of young panicles from five O. sativa, O. rufipogon, O. glaberrima, O. punctata, and O. brachyantha separately. Degradome sequencing (degradome-seq) or parallel analysis of RNA ends (PARE) was carried out using the same samples to analyze miRNA and siRNA targets.

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