NextSeq 500 paired end sequencing; GSM3450452: Column_1 _Control; Mus musculus; RNA-Seq

Column_1 _Control

We use single-cell RNA sequencing (scRNA-seq) to explore the transcriptional changes associated with estrogen-induced dysplasia in mouse ovarian surface epithelial cells Overall design: scRNA-seq of control and estrogen-treated (100nM) mOSE cultured for 15 days. scRNA-seq was performed using the Fluidigm HT 3' RNA-seq protocol on the Fluidigm C1

Single-cell RNA sequencing reveals transcriptional dynamics of estrogen-induced dysplasia in the ovarian surface epithelium

Submitted on 01-NOV-2018

Single cell suspensions of control and E2-treated mOSE were processed using the Fluidigm HT 3' scRNA-seq protocol. The left cell inlet of the integrated fluidics circuit (IFC; leading to columns 1-10 of the plate) were loaded with control cells, while E2-treated cells were loaded into the right inlet (columns 11-20 of the IFC plate) Library were constructed using the standard protocol described for the Fluidigm HT 3' scRNA-seq. Libraries were sequenced on a NextSeq500 high-out 26x75bp run

Single-cell RNA sequencing reveals transcriptional dynamics of estrogen-induced dysplasia in the ovarian surface epithelium

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