Transcriptional profiling of pig striatum after deep hypothermic cardiac arrest

The goal of the study was to identify the gene changes that occur in the striatum of neonatal piglets exposed to cardiopulmonary bypass (CPB) with deep hypothermic cardiac arrest (DHCA) then recovered for 6h. To accomplish our goal, mRNA-seq was performed on RNA isolated from neonatal pigs exposed to either DHCA or sham conditions. Overall design: RNA samples were isolated from the striatum from the brains of 6 neonatal piglets exposed to sham conditions were used as the controls; while samples from 6 piglets exposed to DHCA of 18o for 30 min then recovered for 6h were the experimental group.

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Tu LN, Timms AE, Kibiryeva N, Bittel D, Pastuszko A, Nigam V, Pastuszko P