Our research describe the influence of aeration conditions in petri dishes for A. thaliana growth. We analyze the difference between plants grown in standard Petri dish (Non-aerated) and modified Petri dish that include aeration (Aerated). To characterize the differences between those conditions the gene expression analysis was performed. We also wanted to analyze the effect of using a micropore filter, so we designed another experiment with Aerated, Non-aerated and Micropore filter conditions. Overall design: In the first set there are a total of 8 biological samples. 4 biological replicates each of Aerated (control) and Non-aerated. Additionally in the second set, there are 4 biological replicates of Aerated, Non-aerated and Micropore sealed conditions.