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Accession IconSRP162273

Peripheral Blood miRNA Transcriptomic Analysis for Salmonella Infection in Pig

Organism Icon Sus scrofa
Sample Icon 6 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Peripheral blood transcriptome is an important intermediate data source for investigation of the mechanism of Salmonella invasion, proliferation, and transmission but its development in pig is quite limited. We challenged four-week-old piglets (Duroc × Landrace × Yorkshire crossbred) with Salmonella enterica serovar Typhimurium LT2 and investigated the peripheral blood miRNA expression profile before treatment (d0) and at 2 days post inoculation (dpi) using deep sequencing technology. Overall design: Six healthy piglets (2 groups × 3 replicates) that were challenged with Salmonella. Peripheral blood was collected from the pig and miRNA transcriptome at day 0 and 2 days post infection was determined by Hiseq 2000.
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