Chicken IRF7 knockout by CRISPR-Cas9 in DF-1 and in vitro H6N2 infection followed by RNA-seq

To elucidate the functional role of chicken IRF7 against avian influenza virus (AIV) infection, we generated inducible IRF7 knockout DF-1 cell lines and performed in vitro infection using low pathogenic AIVs (LPAIVs) followed by RNA-seq. Overall design: Transcriptiome analysis of low pathogenic influenza virus (H6N2) infected chicken IRF7 knockout DF-1 cell lines and control cell line at different timeponts (0, 2, 6, 12hpi)

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Kim TH, Kern C, Zhou H