HiSeq X Ten paired end sequencing; GSM3320040: wild-type untreated; Caenorhabditis elegans; RNA-Seq

wild-type untreated

We discovered 5446 genes expression change (4218 genes from P0 to F2) in wild-type C.elegans transcriptome compared to DAMT-1 mutant upon mitochondrial stress by RNA-seq, which indicated those genes response to mitochondrial stress and may function in mitochondrial stress response. Overall design: Wild-type worms or damt-1 mutant were treated by mitochondrial inhibitor antimycin A from L1 to L4, then performed to NGS.

Transcriptome wide change upon mitochondial stress in C.elegans

Submitted by Gene Expression Omnibus on 28-SEP-2018

worm total RNA was extracted by phenol: chloroform: isopentanol (25:24:1) extraction. RNA libraries were prepared for sequencing using Illumina (E7370L) protocols.