The dominant and poor penetrant phenotypes of the maize mutation Unstable factor for orange1 are caused by DNA methylation changes at a linked transposon

Transcriptional analysis of the Ufo1-1 mutant allele and wild type plants were used to identify the candidate gene using a k-mer based approach. The transcriptome data also revealed the altered expression of carbohydrate metabolic genes, which translates into elevated levels of soluble sugars in leaves and light-induced lesions found in mature Ufo1-1 leaves. Overall design: Genome-wide RNA and sRNA profiling was done by Illumina TruSeq sample preparation followed by high-throughput sequencing with Illumina HiSeq 2000 platform. 

27

Wittmeyer K, Cui J, Chatterjee D, Lee TF, Tan Q, Xue W, Jiao Y, Wang PH, Gaffoor I, Ware D, Meyers BC, Chopra S