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Accession IconSRP154372

Differential gene expression in NPHS2-Cre +/+ mouse glomeruli versus wild-type control

Organism Icon Mus musculus
Sample Icon 4 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
To investigate differential gene expression that might account for the differing glomerular phenotype of NPHS2-Cre +/+ mice when compared with wild-type control, including altered GBM thickness, loss of normal foot process morphology, and decrease in podocyte number, RNA sequencing analysis was performed on glomeruli extracted from both NPHS2-Cre +/+ and wild-type control mice. Overall design: Following isolation of glomeruli using Dynabeads from NPHS2-Cre +/+ and wild-type control mice (n=2 biological replicates per genotype, singly isolated), total RNA was extracted and RNA samples were submited for sample preparation and sequencing.
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4
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