RNA-sequencing of Control and TLE3-deficient Beige Adipocytes

Prolonged cold exposure stimulates the recruitment of beige adipocytes within white adipose tissue. Beige adipocytes depend on mitochondrial oxidative phosphorylation to drive thermogenesis. The transcriptional coregulator TLE3 inhibits mitochondrial and metabolic gene expression in beige adipocytes. Overall design: mRNA profiles of iWAT immortalized preadipocytes, differentiated in culture, and knocking out TLE3 after differentiation, were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.

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Pearson S, Loft A, Rajbhandari P, Simcox J, Lee S, Tontonoz P, Mandrup S, Villanueva CJ