Analysis of Wild Type and Leptin-overexpressing Pig PBMC Transcriptomes

Purpose: To find the difference between leptin-overexpressing and wt pig. Methods: Peripheral blood mononuclear cells (PBMCs) mRNA profiles of 6-month-old wild-type (WT) and leptin-overexpressing pig were generated by deep sequencing. Sequencing libraries were constructed according to the protocol for the Illumina TruSeq Sample preparation kit. Sequencing was performed on the Illumina X Ten sequencer. Library construction and sequencing were performed at the Genergy Biotech Co., Ltd. (Shanghai). Results: RNA-seq was performed to detect the transcriptomic changes in PBMCs in the transgenic leptin pigs. With 2-folds change and p<0.05 threshold, we found that 84 transcripts were upregulated, and 124 transcripts were downregulated in the transgenic leptin pigs. Conclusions: The leptin-overexpressing pigs exhibited remarkable resemblance to human SLE in multiple aspects of the disease. Overall design: PBMC mRNA profiles of 6-month-old wild type (WT) and leptin-overexpressing pig were generated by deep sequencing.

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