Illumina HiSeq 4000 paired end sequencing; GSM3229993: L1-1: Stem from low inbred lines; Zea mays; RNA-Seq

L1-1: Stem from low inbred lines

Four samples from the low and high hybrid at big flare period were used for RNA sequencing. Overall design: Four samples from the low and high hybrid at big flare period were used for RNA sequencing.

RNAseq of Maize stem at big flare period

Submitted on 29-JUN-2018

1 μg of total RNA was subjected to library synthesis using the TruSeq V2 RNA-Seq kit.  1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 12 cycles of PCR. Sequencing was performed using 12pM hybridization to a 2x100 paired end flow cell. RNA libraries were prepared for sequencing using standard Illumina protocols