A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti

Here, we present the first whole transcriptome study to address the impact of environmental stress on A. aegypti response to dengue virus. We examined expression profiles of adult females resulting from crowded and optimum reared larvae from the same Trinidad isolate at two critical early time points—3 and 18 hours post dengue virus infected blood meal. We exposed specimens to either a dengue or naïve blood meal, and then characterized the response in ten gene co-expression modules based on their transcriptional associations with environmental stress and time.  Overall design: RNAseq of stressed and optimal dengue and control mosquitoes at 3 and 18 hours post bloodmeal.

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