RNA-seq data from human SGBS adipocytes differentiated with marine oxohexadecenoic acids

We recently isolated and identified (7E)-9-oxohexadec-7-enoic acid (1) and (10E)-9-oxohexadec-10-enoic acid (2) from the marine algae Chaetoceros karianus. Synthesis and biological characterization show that these are PPARa/? dual agonists. Herein we report the gene expression data from human SGBS pre-adipocytes, stimulated to differentiate with 1, 2 or the classical PPAR? agonist rosiglitazone. The transcriptome analysis shows that both compounds induce anti-diabetic gene programs in adipocytes by upregulating insulin-sensitizing adipokines and repressing pro-inflammatory cytokines. Overall design: Human SGBS pre-adipocytes were stimulated with adipogenic media supplemented with either (7E)-9-oxohexadec-7-enoic acid, (10E)-9-oxohexadec-10-enoic acid, or rosiglitazone from day 0 to day 4. On day 4, agonists were withdrawn, and the cells were allowed to differentiate following standard protocol. On day 8, RNA was isolated and sent to sequencing.

12

Sæther T, Paulsen SM, Tungen JE, Vik A, Aursnes M, Holen T, Hansen TV, Nebb HI