Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

The human pluripotent stem cells cultured on Low-adhesion substrates formed two morphologically different subtypes (Monolayer and Domed). The dome-like cells showed higher proliferation capacity and KLF4/5 expression than the monolayer cells. A serum response factor based regulatory double loop was proposed to explain how cell-matrix adhesion mediates the interaction between cell morphology and pluripotency genes Overall design: Compare the two type cells with RNA-seq to investigate the different expression

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Yu L, Li J, Hong J, Takashima Y, Fujimoto N, Nakajima M, Yamamoto A, Dong X, Dang Y, Hou Y, Yang W, Minami I, Okita K, Tanaka M, Luo C, Tang F, Chen Y, Tang C, Kotera H, Liu L