Profiling of murine resident adipose tissue and aortic macrophages according to their expression status of LYVE-1

Beside their role in conventional immune regulation, macrophages are now recognised as essential regulator of local tissue homeostasis depending on the tissue in which they reside. Using phenotyping, we found that LYVE-1+ macrophages are the major resident macrophage population in murine aorta and adipose tissues under steady state. Furthermore, imaging analysis revealed the exclusive association of adipose tissue LYVE-1+ macrophages with smooth muscle positive large blood vessels. Hence, we hypothesize that LYVE-1+ macrophages sustain large vessel functional homeostasis. The present experiment aims to better characterize resident LYVE-1+ vs LYVE-1- macrophages in aorta and adipose tissues. Overall design: LYVE-1+ and LYVE-1- aortic macrophages were FACS sorted as DAPI-CD45+CD64+MerTK+CD11b+F4/80+LYVE-1+ and DAPI-CD45+CD64+MerTK+CD11b+F4/80+LYVE-1- respectively from 30 adult C57/BL6 mice (n =3) their RNA extracted for transcriptome profiling. Similarly, LYVE-1+ and LYVE-1- adipose tissue macrophages were FACS sorted as DAPI-CD45+CD64+MerTK+CD11b+F4/80+LYVE-1+ and DAPI-CD45+CD64+MerTK+CD11b+F4/80+LYVE-1- respectively from 20 adult C57/BL6 epididymal and subcutaneous adipose tissue (n =3) their RNA extracted for transcriptome profiling.

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Lim HY, Lim SY, Tan CK, Thiam CH, Goh CC, Carbajo D, Chew SHS, See P, Chakarov S, Wang XN, Lim LH, Johnson LA, Lum J, Fong CY, Bongso A, Biswas A, Goh C, Evrard M, Yeo KP, Basu R, Wang JK, Tan Y, Jain R, Tikoo S, Choong C, Weninger W, Poidinger M, Stanley RE, Collin M, Tan NS, Ng LG, Jackson DG, Ginhoux F, Angeli V