Integrated analysis of lncRNA and mRNA reveals the temporal expression patterns during chicken brain development and aging

We systematically investigated the lncRNA and mRNA temporal expression profile of the female chicken brain by high-throughput sequencing 8 stages across their entire lifespan. We identified and classified 39,907 putative lncRNAs, and predicted the potential biological functions of lncRNAs based on WGCNA. Temporal expression patterns were investigated based on a set of age-dependent genes, results showed that genes functioned in development, synapse and axon exhibited a progressive decay; genes related to immune response were up-regulated with age, And some genes showed inversion of their temporal profiles. These results demonstrated dynamic changes in lncRNA and mRNA with age, which may reflect changes in regulation of transcriptional networks and provides non-coding RNA gene candidates for further studies. It would be vital significance in avian epidemic prevention and contribute to comprehensively understand the molecular mechanisms of chicken breeding and reproduction. Besides, birds, as important species to bridge the evolutionary gap between mammals and other vertebrates, would contribute to further improve the understanding of the role in evolution. Overall design: rRNA-deleted RNA-Seq was performed on chicken brain to profile the lncRNAs and mRNAs across the entire life span

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Xu Z, Che T, Li F, Tian K, Zhu Q, Mishra SK, Dai Y, Li M, Li D